Mathieu SCHMITT

 [email protected]  http://m.schmitt.pharma.free.fr

26 years, single, French nationality

Neuro-Scientist WORK EXPERIENCE Scientist in Neuroscience and Pharmacology

Sept. 2010 - Jan. 2012

UCB pharma S.A., Braine l’Alleud, Belgium. Team New Targets Biology, department Central Nervous System Biology 

Research on the functionality of neuronal and synaptic target proteins and optimization of functional assays to find a proof of concept in in vitro models

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Study of synaptic neurotransmission, neurodegeneration, and signaling pathway implicated in Parkinson's and Alzheimer's diseases and in epilepsy

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Collaborative works with the bioinformatics in order to optimize analysis processes of results and images to obtain high content screening assays for drugs identification

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Project management, analysis and results presentation, training and technology transfer

Scientist internship in neuro-pharmacology of Master

Feb. 2010 – Sept. 2010

UCB pharma S.A., Braine l’Alleud, Belgium. Team New Targets Biology 

Characterization of in vitro functional system for identification of active compounds in the mechanism of synaptic transmission

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Study on exo-endocytosis mechanism of synaptic vesicle involved in the synaptosomal fractions

Technician in management of experimental breeding

(3 months) 2009

CNRS UPR 3212, Institute of Cellular and Integrative Neurosciences, Strasbourg, France 

Management of the reproduction of rats Sprague-Dawley and transgenic mice C57Bl6

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Technical management, installation of the orders, disinfection and observations of research

Order picker, Puma France S.A.S., Illkirch, France Production technician in food processing, Herta S.A., Illkirch, France Warehouseman, Puma France S.A.S., Illkirch, France

(2 months) 2008 (1 month) 2007 (2 months) 2006

Assistant in car accident service, Insurance of Crédit Mutuel S.A.S., Strasbourg, France (2 months) 2005

EDUCATION Master of Sciences, Technology and Health, specialized Pharmaceuticals Sciences, option Pharmacology with honors

2010

University of Strasbourg, Faculty of Pharmacy, Strasbourg, France 

Practical lab course : pathway of protein Rab 5 in the membrane internalization (CNRS 7213)

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Certificate of animal experimentation level 1

Licence / Bachelor of Sciences, specialized in Life Sciences, option Cell Biology and Physiology

2008

University Louis Pasteur, Faculty of Life Sciences, Strasbourg DEUG Sciences, specialized in Life Sciences, option in Biochemistry and Molecular Biology

2007

University Louis Pasteur, Faculty of Life Sciences, Strasbourg First Year of Medicine Studies, PCEM1

2005

University Louis Pasteur, Faculty of Medicine, Strasbourg Scientific French baccalaureate, equivalent to 'A' levels, specialized in Earth and Life Sciences

2004

Jean Rostand high school, Strasbourg

Mathieu Schmitt,  [email protected] –  m.schmitt.pharma.free.fr

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SKILLS Fundamental knowledge 

Molecular, experimental, cellular and integrative pharmacology, in vitro and in vivo models, toxicology, physiopathology, pharmacokinetics, clinical trials, pharmacognosy, function and mechanism of synaptic protein, etc. Personal and social aptitudes

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Ability to adapt to a new working environment and to suggest innovative solutions that can help solving problems

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Enthusiasm, flexibility and autonomy

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Excellent communication aptitude

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Perseverance to find solutions, vigorous and ready to take up the challenge Organizational skills and project management

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Management, coordination and planning of experimental research projects. Design and working-out of protocols and optimization of experimental procedures

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Theoretical and statistical analysis of experimental results. Writing, presenting and highlighting of activity reports, data or scientific posters

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Management of laboratory and of research budget : taking orders, managing stocks and technical/biological material maintenance

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Bibliographic, scientific and technologic monitoring

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Aptitude of planning and working in collaboration

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Aptitude to impart information, knowledge and protocol by doing supervision and training In vitro research techniques

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Development and optimization of in vitro functional assays for study and screening compound libraries

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Identification and improvement of model to study new pharmacological targets

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Expertise in cell culture techniques (HEK, CHO, SH-SY5Y, etc.), management of cell line stocks (starting cell culture, subculturing, freezing), aseptic and decontamination techniques

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Culture of primary cortical or hippocampal neurons from embryonic rat

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Experienced in confocal fluorescent microscopes and high content screening  Cellomics array Scan VTI (high content bioimager, Thermo Scientific)  Pathway 855 (high content bioimager, Becton Dickinson)  Leica TCS SP5 II DMI6000B (confocal microscope,Leica Microsystem)  Zeiss Observer Z1 (fluorescent microscope, Carl Zeiss)

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Image analysis for high content assays  Definiens Architect et Developer v1.2  BD Attovision v1.6.2

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Experienced in fluorometric instruments for medium content screening  Flexstation II et III (Molecular Device) and platereader  Celllux (Perkin Elmer)

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Experienced in flow cytometers (FACSflow)  FACSCANTO II (Becton Dickinson)

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Experienced in molecular biology for protein study  Western blot  Immunocytochemistry  PCR

Mathieu Schmitt,  [email protected] –  m.schmitt.pharma.free.fr

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Expertise in cell calcium signaling field by measurement of ratiometric and non-ratiometric fluoromectic signal (Fura-2, Fluo-4)

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Knowledge of functional methods to study the mechanism of exo-endocytosis by Fm dye fluorescent marker of the synaptic vesicles

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Preparation by purification of synaptosomes and synaptic vesicles from rat brain In vivo research techniques

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« Certificat d’expérimentation animale de niveau 1 » (certificate animals experimentation level 1, official French certificate)

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Management of animal house, orders, disinfection techniques, breeding process in standard protocols of rat and mice reproduction (supervision of coupling, gestation, weaning, animal identification, cage change, etc.)

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Animal wellness, facilitate the conduct of ethical and regulation-compliant research, hygiene and security

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Basic grounding of development and optimization behavioral techniques (Morris swimming pool, passive avoidance, Y-maze, etc.)

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Knowledge of chirurgical techniques on rat and mice : vasectomy, ovariectomy, surrenalectomy, organ sampling, brain sampling (cortex, hippocampus, cerebellum, striatum), embryo sampling and anaesthesia techniques

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Basic knowledge in blood sampling techniques, force-feeding and intravenous in rodent Informatics, data and statistical analysis

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Quantitative analysis of microscope images by computer-assisted detection using Definiens XD v1.2 software (detection of neurite outgrowth, puncta, object intensity, nuclear translocation, etc.)

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Research and use of scientific resources on internet (Pubmed, NCBI, EMBL, PDBe, etc.)

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Experienced in informatics software, management and data analysis 

Office :

Microsoft Office Word, Excel, PowerPoint, Access, v2010, OpenOffice v3.4.0

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Morphometry :

BD Attovision v1.6.2, Definiens Viewer, Developer and Archictect XD v1.2, Syngène

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Statistics :

Graphpad Prism v5.2, Tibco Spotfire v3.2, IDBS Excelfit v5.2, Systat SigmaPlot v12.2

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Instrument :

Softmax pro v5.4.1, BD Attovision v1.6.2, BD FACS Diva v6.0

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Graphism :

Adobe Photoshop CS6 v13, Paint Shop Pro vX, imageJ v1.4, GIMP v2.6

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Bibliographies :

Endnote X5, Refmanager v12, Mendeley v1.5.2

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Database :

Itemtraker, Nextbio, Pipeline Informa Healthcare, Ingenuity

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Website :

HTML, CSS and PHP, Webexpert v6, Dreamweaver CS5 v12, Notepad++ v6.1.5

Languages 

English :

good comprehension, written and oral expression

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German :

school level

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French :

native tongue

MISCELLANEOUS Hobbies : swimming, jogging, hiking, tennis, gardening, cooking, pastries and cakes baking Driving license and car owner

Mathieu Schmitt,  [email protected] –  m.schmitt.pharma.free.fr

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