supplementary material - Bentham Open

123-145. 139-175. 141-190. 118-122. 121-127. 130-164. 184-208. 0.32≤He≤0.84. 0.07. 0.85. 0.47 ...... J Evol Biol 2004; 17: 363-75. [52]. Natoli A, Birkun A, ...
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Supplementary Material

The Open Marine Biology Journal, 2008, Volume 2

SUPPLEMENTARY MATERIAL Table A. Dinucleotide Microsatellite Loci Isolated or Amplified in Cetaceans from 1989 to 2007

Locus Information

Species

Number of Individuals Tested

Number of Different Alleles (Na)

(TC)n repeat

Globicephala melas*

11

3

Size Range (or Size of the PCR Product) 114-132

He

Ho

Reference

[1]

primers: TGAAATTCTTCATCAGT GTTAATGTAGGCAGACT 199/200, (CT)n repeat

Globicephala melas*

A

GenBank : X68820

Tursiops truncatus

A

primers :

Orcinus orca

A

TGAAATTCTTCATCAGT

Lagenorhynchus acutus

A

GTTAATGTAGGCAGACT

Phocoena phocoena

A

Physeter macrocephalus

A

Hyperoodon ampullatus

A

Balaenoptera borealis

[2]

A

Balaenoptera physalus

8

4

Balaenoptera acutorostrata

10

3

Globicephala melas

193

5

Megaptera novaeangliae

1402

9

Globicephala melas

529

5

Megaptera novaeangliae

142

10

103-121

0.608

0.636

[6]; see also [7]

Stenella coeruleoalba

97

15

106-134

0.82

0.77

[8]

Tursiops spp.

305

8

114-134

0.749

0.717

[9]; see also [10]

0.598He0.614

0.578Ho0.619

110-134

[4] 0.212He0.465

Megaptera novaeangliae

648

9

102-118

409/470, (GT)n or (GA)n repeat

Globicephala melas*

193

8

174-188

primers :

Globicephala melas

529

9

GTTTTGGTTGCTTGA TAAAAGACAGTGGCA

[3] [5]

[11] [3]

0.499He0.674

[5]

i

ii The Open Marine Biology Journal, 2008, Volume 2

Supplementary Material (Supplementary Table A) contd.....

Number of Individuals Tested

Number of Different Alleles (Na)

Locus Information

Species

415/416, (TG)n repeat

Globicephala melas*

A

GenBank : X68821

Tursiops truncatus

A

primers :

Orcinus orca

A

GTTCCTTTCCTTACA

Lagenorhynchus acutus

A

Physeter macrocephalus

A

Hyperoodon ampullatus

A

ATCAATGTTTGTCAA

Balaenoptera borealis

GenBank : X68822 primers : GTGATATCATACAGTA ATCTGTTTGTCACATA

He

Ho

Reference

[2]

A

Balaenoptera physalus

8

A

Balaenoptera acutorostrata

10

3

Globicephala melas

193

6

222-234

Phocoena phocoena

124

9

202-230

Megaptera novaeangliae

65

2

227-229

Globicephala melas

529

5

Eubalaena spp.

417/418, (TG)n repeat

Size Range (or Size of the PCR Product)

[3] [12] 0.04He0.22

0.04Ho0.24

0.516He0.624

[5]

-

Stenella longirostris

132$

Phocoena phocoena Globicephala melas* Tursiops truncatus Orcinus orca Lagenorhynchus acutus Phocoena phocoena Physeter macrocephalus Hyperoodon ampullatus Balaenoptera borealis Balaenoptera physalus Balaenoptera acutorostrata Globicephala melas Phocoena phocoena Megaptera novaeangliae Globicephala melas Eubalaena spp. Stenella coeruleoalba Megaptera novaeangliae

77

8 10 193 124 1399 529 99 648

[14]

12 A A A A A A A A 5 4 3 8 9 4 15 12

[13]

0.833

0.788

[15]

0.22He0.55

0.24Ho0.52

[16] [2]

181-187 162-180 0.396He0.510 161-195 176-204

0.69 0.818He0.838

0.63 0.775Ho0.844

[3] [12] [4] [5] [14] [8] [11]; see also [6, 7]

Supplementary Material

The Open Marine Biology Journal, 2008, Volume 2

iii

(Supplementary Table A) contd.....

Number of Individuals Tested

Number of Different Alleles (Na)

Locus Information

Species

464/465, (TG)n repeat

Globicephala melas*

A

GenBank : X68823

Tursiops truncatus

A

primers :

Orcinus orca

A

GGAAATGCTCTGAGAAGGTC

Lagenorhynchus acutus

A

CCAGAGCACCTATGTGGAC

Phocoena phocoena

A

Physeter macrocephalus

A

Hyperoodon ampullatus

A

Balaenoptera borealis

Size Range (or Size of the PCR Product)

He

Ho

Reference

[2]

A

Balaenoptera physalus

8

3

Balaenoptera acutorostrata

10

4

Globicephala melas

193

8

138-154

Megaptera novaeangliae

68

6

137-151

Megaptera novaeangliae

1443

6

Delphinapterus leucas

640

6

Globicephala melas

529

7

Delphinapterus leucas

1300

6

130-142

Megaptera novaeangliae

648

8

132-152

468/469, (GT)n or (GA)n repeat

Globicephala melas*

193

54

87-185

primers :

Globicephala melas

529

72

EV1Pm, (AC)n repeat

Physeter macrocephalus*

10

6

GenBank : G09074

Pontoporia blainvillei

1

-

primers :

Delphinapterus leucas

19

4

CCCTGCTCCCCATTCTC

Monodon monoceros

1

1

ATAAACTCTAATACACTTCCTCCAAC

Phocoena phocoena

10

3

Steno bredanensis

2

-

Sotalia fluviatilis

2

-

Lagenorhynchus albirostris

2

-

Lagenorhynchus obscurus

1

-

[3] 0.58He0. 65

0.56Ho0.78

[13] [4]

130-142

0.056He0.718

[17]

0.652He0.694 0.574He0.581

[5] 0.56

[18]

0.544Ho0.580

[11]; see also [7, 19] [3]

0.932He0.978

[5]

ACCCCAGAGAAAACA CAAGGTATTTCAGAA 115-197#

[20]

iv

The Open Marine Biology Journal, 2008, Volume 2

Supplementary Material

(Supplementary Table A) contd.....

Locus Information

Size Range (or Size of the PCR Product)

Species

Number of Individuals Tested

Number of Different Alleles (Na)

Lagenorhynchus acutus

5

1

Tursiops truncatus

10

8

Stenella frontalis

1

-

Stenella coeruleoalba

10

5

Delphinus delphis

8

-

Globicephala melas

400

8

Orcaella brevirostris

1

1

Berardius bairdii

1

-

Mesoplodon bidens

7

4

Ziphius cavirostris

1

-

Kogia simus

1

-

Eschrichtius robustus

1

2

Balaenoptera musculus

1

-

Physeter macrocephalus

158

11

Tursiops aduncus

20

4Na9

0.32He0.84

Balaenoptera physalus

64

12

0.07

0.36

[25]

Eubalaena australis

29

9

0.85

0.84

[14]

Eubalaena glacialis

30

4

0.47

0.44

Balaenoptera acutorostrata

306

14

139-175

0.817He0.844

0.826Ho0.870

[26]

Tursiops spp.

305

21

141-190

0.846

0.864

[9]; see also [10, 27]

Lagenorhynchus obliquidens

59

3

118-122

0.54

0.59

[28]

Megaptera novaeangliae

648

4

121-127

0.551He0.553

0.531Ho0.556

[11]; see also [29, 4, 6, 19, 7, 30]

Balaenoptera borealis

89

14

130-164

0.806

Hyperoodon ampullatus

180

12

184-208

0.833He0.845

0.943Ho1.000

[32]

Eubalaena glacialis Balaena mysticetus Balaenoptera brydei Tursiops aduncus Stenella longirostris Grampus griseus

278 134 508 76 136$ 51

0.484 6 >10 7 15 18

0.447 0.756 0.413