MobyleNet - ` `%%%`#`&12_`__~~~ alse - Maupetit Julien

Nov 30, 2009 - Search for a 3D template. Select templates of interest. Query/template sequence alignment. Preparing modeller input. Perform the 3D modeling.
14MB taille 3 téléchargements 212 vues
The Mobyle Network

Tutorial - Homology modeling pipeline

MobyleNet http://mobylenet.rpbs.univ-paris-diderot.fr

Julien Maupetit, Pierre Tufféry. -RPBS2009/11/13

The Mobyle Network

1

The Mobyle Network

Tutorial - Homology modeling pipeline

The Mobyle Network

1

The Mobyle Network

2

Tutorial - Homology modeling pipeline

Tutorial - Homology modeling pipeline

The Mobyle Network

The Mobyle Network

Tutorial - Homology modeling pipeline

The Mobyle Network

Tutorial - Homology modeling pipeline

Principles Institut Pasteur RPBS IGR MSG

Bioinformatics - Molecular modelling Bioinformatics - Genomic Structural biology MicroArrays

Rennes

GenOuest

MobyleNet

Toulouse

Confidence network Each portal can import/export a specific service to/from a specific Mobyle portal

Nice

Montpellier

Université Nice LIPM

Distributed mobyle portals Each portal has its own specificity (banks and programs)

Paris

CBS

User centered Single interface for multiple portals

→ Service maintainers are service deployers

The Mobyle Network

1

The Mobyle Network

2

Tutorial - Homology modeling pipeline Synopsis Step by step tutorial Remote search for sunflower protein sequence Search for a 3D template Select templates of interest Query/template sequence alignment Preparing modeller input Perform the 3D modeling A quick glance at the 3D model Any further analysis ?

Summary

Tutorial - Homology modeling pipeline

The Mobyle Network

Tutorial - Homology modeling pipeline

Synopsis We illustrate here how to take advantage of the MobyleNet to model the 3D structure of the sunflower protein corresponding to the HuCL01857C002 genomic information.

tools at the three nodes (LIPM, Pasteur and RPBS) can be chained to complete the modeling nota bene : all calls are performed within the RPBS (remote imported services) portal

The full tutorial is available at : http://mobylenet.rpbs.univ-paris-diderot.fr/doc/tutorials/network.html

The Mobyle Network

Tutorial - Homology modeling pipeline

Remote search for sunflower protein sequence

The Mobyle Network

Tutorial - Homology modeling pipeline

Remote search for sunflower protein sequence

The Mobyle Network

Tutorial - Homology modeling pipeline

Remote search for sunflower protein sequence

The Mobyle Network

Tutorial - Homology modeling pipeline

Remote search for sunflower protein sequence

The Mobyle Network

Tutorial - Homology modeling pipeline

Remote search for sunflower protein sequence

The Mobyle Network

Tutorial - Homology modeling pipeline

Remote search for sunflower protein sequence

The Mobyle Network

Tutorial - Homology modeling pipeline

Remote search for sunflower protein sequence

The Mobyle Network

Search for a 3D template

Tutorial - Homology modeling pipeline

The Mobyle Network

Search for a 3D template

Tutorial - Homology modeling pipeline

The Mobyle Network

Search for a 3D template

Tutorial - Homology modeling pipeline

The Mobyle Network

Search for a 3D template

Tutorial - Homology modeling pipeline

The Mobyle Network

Select templates of interest

Tutorial - Homology modeling pipeline

The Mobyle Network

Select templates of interest

Tutorial - Homology modeling pipeline

The Mobyle Network

Query/template sequence alignment

Tutorial - Homology modeling pipeline

The Mobyle Network

Query/template sequence alignment

Tutorial - Homology modeling pipeline

The Mobyle Network

Query/template sequence alignment

Tutorial - Homology modeling pipeline

The Mobyle Network

Preparing modeller input

Tutorial - Homology modeling pipeline

The Mobyle Network

Preparing modeller input

Tutorial - Homology modeling pipeline

The Mobyle Network

Preparing modeller input

Tutorial - Homology modeling pipeline

The Mobyle Network

Preparing modeller input

Tutorial - Homology modeling pipeline

The Mobyle Network

Perform the 3D modeling

Tutorial - Homology modeling pipeline

The Mobyle Network

Perform the 3D modeling

Tutorial - Homology modeling pipeline

The Mobyle Network

Perform the 3D modeling

Tutorial - Homology modeling pipeline

The Mobyle Network

A quick glance at the 3D model

Tutorial - Homology modeling pipeline

The Mobyle Network

A quick glance at the 3D model

Tutorial - Homology modeling pipeline

The Mobyle Network

A quick glance at the 3D model

Tutorial - Homology modeling pipeline

The Mobyle Network

Any further analysis ?

Tutorial - Homology modeling pipeline

The Mobyle Network

Any further analysis ?

Tutorial - Homology modeling pipeline

The Mobyle Network

Tutorial - Homology modeling pipeline

Summary

HuCL01857C002

1 LIPM

HeliageneGetSequence

2 RPBS 3

PDBBlast2

XMLBlastSeq

3D Model

6 5

Modeller

Ali2Modeller

4 Pasteur

Clustalw-multialign