Product Specification Sheet

FT500-ND PULSE™ Tube …the PCT Sample Preparation System Pressure Cycling Technology (PCT) uses rapid cycles of hydrostatic pressure between ambient and ultra high levels to control biomolecular interactions, allowing for a high degree of safety, speed, reproducibility, and convenience. This unique, patented technology offers the potential for broad applications in a number of established and emerging fields, including genomics, proteomics, pathogen inactivation, drug discovery and development, protein purification, and immunodiagnostics. Sample preparation is a significant bottleneck to discoveries in genomic and proteomic research. To address this problem, the PCT Sample Preparation System (PCT SPS) was developed. The System allows for the safe, rapid, and reproducible extraction of DNA, RNA, proteins, and small molecules from a wide variety of cells and tissues, particularly those considered “hard-to-lyse”. In addition to extraction, the PCT SPS can be used to accelerate enzymatic reactions under pressure. The PCT SPS uses a small, semi-automated bench top instrument (Barocycler NEP3229 or the NEP2320) in combination with single-use, application specific, sample processing containers called PULSE Tubes. FT500-ND PULSE Tube

Unlike the FT500 PULSE Tube, the FT500-ND does not have a Lysis Disk. It was designed for processing solutions, suspensions, or complex matrices – such as soil – that do not require passage through a Lysis Disk for partial homogenization. A Blue Ram is first set into place using the provided PULSE Tube Tool. The sample and processing buffer are then added through the Cap end of the PULSE Tube and the Sample Chamber is closed by inserting the Blue Cap. Since there is no Lysis Disk, greater variation in both sample amount and processing buffer volume is possible than with the FT500 (researchers should consult specific applications for the use of the FT500-ND). Like the FT500, the FT500-ND transmits the power of PCT (pressure) from the Barocycler instrument to the sample. The assembled FT500-ND is placed in the pressure chamber of the Barocycler, PCT conditions are selected, and pressure cycling begins. During the PCT process, the Ram compresses the sample and processing buffer against the inside of the Blue Cap, resulting in pressure being transmitted to the sample. When one PCT cycle has finished, the Ram partially retracts, as pressure is released. This process is repeated for the requisite number of pressure cycles for a particular application. The combination of rapid pressure changes, chemistry, and other bio-physical mechanisms, together with the new diskless FT500ND, make this new PULSE Tube ideal for processing bacteria, complex matrices, extracting mitochondria from cells, or for accelerating trypsin digestion and other enzymatic reactions under pressure.

Specifications of the FT500-ND PULSE Tube* Dimensions

Material Sample Size**

13 mm diameter × 51 mm long Sample Chamber: Polypropylene Blue Cap & Ram: Polyethylene O-ring Seal: silicon rubber Solid: 50-300 mg Liquid: 0.2-1.4 ml

Compatible solvent

Operating Temperature*** Storage Temperature

Solvent that is compatible with polypropylene, polyethylene and silicon rubber 4 to 60°C - 20 to 35 °C

* Specifications may be changed without prior notification ** Consult specific applications for sample size and volume requirements *** Autoclave recommended method: Standard liquid setting

FT500-ND Diagram

Cap

Sample Chamber Ram Features & Benefits of the FT500-ND

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Versatile Range of Sample Sizes

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Versatile Range of Buffer Volumes

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Process Solutions & Suspensions

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Extract Bacteria and Fungi in Soil

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Extract Mitochondria from Cells

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Accelerate Proteolytic Digestions for Mass Spectrometry

Publications & Protocols Schumacher RT et al. (2002). “Automated Solution for Sample Preparation: Nucleic Acid and Protein Extraction from Cells and Tissues Using Pressure Cycling Technology (PCT). Am. Laboratory 34, 38-43”. López-Ferrer, D., Petritis, K., Hixson, K.K., Heibeck T.H., Moore, R.J., Belov, M.E., CampII, D.G., and Smith, R.D. (2008) “Application of Pressurized Solvents for Ultrafast Trypsin Hydrolysis in Proteomics: Proteomics on the Fly”, Journal of Proteome Research, ASAP J. Proteome Res., ASAP Article, 10.1021/pr7008077 Web Release Date: July 8, 2008 Shane A. Wyatt and Timothy R. Croley (2008) “High Pressure Trypsin Digestion of Proteins for Proteomic Analysis”, Poster at th 56 Annual ASMS Conference on Mass Spectrometry, June 5, 2008, Denver, CO Remsen, T., Momeni, M., Kessler, P., Francois, F., Stern, A., Anand, S., and Pevsner, P., (2008) “IMAGING MALDI of Colorectal Carcinoma - Field Defects in Satellite Tissue”, American College of Gastroenterology Meeting 2008, MARM 2008, May 20, 2008, Queensborough Community College, Queens, NY PBI Protocol Soil-PrEP PBI Protocol Mitochondria (Cell)-PrEP PBI Protocol SkinTape-PrEP PBI Protocol Proteolysis-PrEP V1. 072808

Pressure BioSciences, Inc. 14 Norfolk Ave, South Easton, MA 02375 TEL 508-230-1828 • FAX 508-230-1829 www.pressurebiosciences.com