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Oligonucleotides: used for priming and should be at least 20-24 nucleotides in length. ○ Standard Buffer for PCR. ○ Taq DNA polymerase: this enzyme purified ...

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Discussion. The chromatogram presented was done using a Cogent Diamond column and an ANP gradient. The addition of weak acetic acid to the.

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212, 457–468. Ratech, H. (1992). Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain ...

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