Ludovic CARLIER To contact me, please visit: http://www.ludoviccarlier.com Nationality: French Date of birth: 12/29/1977
Educational Background 2002/Jul2006 Degree of doctor in Structural Biology (PhD). Biological Nuclear Magnetic Resonance. University of Rouen, France. 2001/2002
Master degree in Biological NMR and Mass Spectroscopy University of Evry Val d’essonne, France. (ranking: 3rd/12).
2000/2001
Maîtrise in Chemistry (equivalent to first year Master degree). University of Rouen, France.
1997/2000
Bachelor degree in Chemistry University of Rouen, France.
Professional experience
Research: 2007/2008
Postdoctoral fellowship: “Using NMR and computational methods to support structure-based drug design on phosphatases”. Bijvoet Center for Biomolecular Research, NMR Spectroscopy Research Group (Pr. Rolf Boelens), Utrecht, The Netherlands. Project advisors: Pr. Rolf Boelens and Dr. Alexandre Bonvin
2002/2006
Thesis work: “Recombinant production of human proline dehydrogenase domains for structural study and structural characterization of region 51-160 of human KIN17 protein by NMR in solution and Molecular Modelling”. Laboratory of Nuclear Magnetic Resonance (Pr. Daniel Davoust), IRCOF, University of Rouen, France. Work in collaboration with the Laboratory of Protein Structure (Dr. Bernard Gilquin), DIEP, CEA of Saclay, France. Thesis advisor: Pr. Daniel Davoust
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2002
Master research course: “Structural study of human urotensin II cyclopeptide by NMR and Molecular Modelling”. Laboratory of Nuclear Magnetic Resonance (Pr. Daniel Davoust), IRCOF, University of Rouen, France. Master advisor: Pr. Daniel Davoust
Teaching: 2007/2008
Practical Nuclear Magnetic Resonance taught in English to first- and third-year graduate students (170 hours). University of Utrecht, The Netherlands.
2003/2004
Practical Analytical Chemistry taught in French to first- and second-year graduate students (61 hours). University of Rouen, France.
Practical experience NMR
Acquisition and processing of 2D/3D homonuclear and heteronuclear (1H, 15N, 13C) NMR experiments on high field Bruker spectrometers (assignment, 15N relaxation, and DOSY experiments). Spectra analysis: NMRView, NMRPIPE, CCPN, FELIX, and SPARKY.
Molecular Modelling
Molecular dynamics under NMR restraints: NOE automatic assignment, structure calculation, and docking using CNS, ARIA, CYANA, INCA, and HADDOCK softwares. Visualisation: SYBYL, PyMOL, MolMol.
Biochemistry
Bacterial culture and isotope labelling. Expression of recombinant proteins using heterologous systems (different E. coli strains and fusion partners). Protein expression and bacterial cell lysis with standard large-scale process and microscale process (using microplates). Protein purification: Gel Filtration and Affinity chromatography. Protein analysis: SDS-PAGE, Western blot, Assay of protein concentration (UV absorbance, Bradford assay…).
Molecular Biology
Cloning techniques: PCR, site-specific recombination (Gateway), DNA sequencing. Site-directed mutagenesis techniques.
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Conferences Oral presentation
Poster
Carlier, L., le Maire, A., Braud, S., Masson, C., Gondry, M., Zinn-Justin, S., Guilhaudis, L., Milazzo, I., Davoust, D., Gilquin, B. and Couprie, J. Journée de l’Ecole Doctorale Chimie-Biologie. Rouen (France) 17 March 2006. “Structural characterization of region 51-160 of human KIN17 protein by NMR in solution and Molecular Modelling”. Carlier, L., Braud, S., Gondry, M., Zinn-Justin, S., Guilhaudis, L., Milazzo, I., Campion, D., Frebourg, T. and Davoust, D. 12th Scientific Workshop of the Federate Institute for Multidisciplinary Research on Peptides (IFRMP 23). Le Havre (France) 10 June 2005. “Recombinant production and screening of expression conditions of 4 PRODH domains for structural study”.
Poster
Carlier, L., Chatenet, D., Milazzo, I., Leprince, J., Oulyadi, H., Tonon, MC., Vaudry, H. and Davoust, D. 11th Scientific Workshop of the Federate Institute for Multidisciplinary Research on Peptides (IFRMP 23). Dieppe (France) 4 June 2005. “Structural study of human urotensin II cyclopeptide by NMR spectroscopy: a powerful tool for biomolecules analysis in solution”.
Poster
Chatenet, D., Debussy, C., Leprince, J., Boularan, C., Carlier, L., Milazzo, I., Guilhaudis, L., Oulyadi, H., Scalbert, E., Pfeiffer, B., Renard, P., Pacaud, P., Davoust, D., Lihrmann, I., Tonon, MC. and Vaudry, H. Peptide Receptors. Montréal (Québec) 31 July-4 August 2004. “URP, a novel urotensin-II related peptide: structure-activity relationships and conformational studies”.
Poster
Chatenet, D., Leprince, J., Debussy, C., Boularan, C., Carlier, L., Milazzo, I., Guilhaudis, L., Oulyadi, H., Davoust, D., Scalbert, E., Pfeiffer, B., Renard, P., Pacaud, P., Lihrmann, I., Tonon, MC. and Vaudry, H. 3rd International and 28th European Peptide Symposium. Prague (Czech Republic) 5-10 September 2004. “Structure-activity relationships and structural conformation studies of a novel urotensin II-related peptide”.
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Publications Chatenet, D., Dubessy, C., Leprince, J., Boularan, C., Carlier, L., Milazzo, I., Guilhaudis, L., Oulyadi, H., Davoust, D., Scalbert, E., Pfeiffer, B., Renard, P., Tonon, MC., Lihrmann, I., Pacaud, P. and Vaudry, H. (2004). Structure-activity relationships and structural conformation of a novel urotensin II-related peptide. Peptides, 25, 1819-1830. Carlier, L., le Maire, A., Braud, S., Masson, C., Gondry, M., Zinn-Justin, S., Guilhaudis, L., Milazzo, I., Davoust, D., Gilquin, B. and Couprie, J. (2006). NMR assignment of region 51-160 of human KIN17, a DNA and RNA-binding protein. Journal of Biomolecular NMR, 36 Suppl 1:29. Carlier, L., Couprie, J., le Maire, A., Guilhaudis, L., Milazzo-Segalas, I., Courçon, M., Moutiez, M., Gondry, M., Davoust, D., Gilquin, B. and Zinn-Justin, S. (2007). Solution structure of the region 51-160 of human KIN17 reveals an atypical winged helix domain. Protein Science, 16, 2750-2755.
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