ANNE-LAURE BOUGÉ PROJECT LEADER PHD. in biochemistry, cellular and molecular biology With over 14 years experience in management of scientific projects in the field of molecular genetics and epigenetics, I would like to lead a dynamic and innovative team in molecular diagnostics.
Work history PROJECT MANAGER: Institute of Regenerative Medicine and Biotherapy
2017
Team Bioinformatics and biomarkers Project: Identification of chimeric RNAs as potential molecular biomarkers for Acute Myeloid Leukemia diagnosis and prognosis.
118 rue Chico Mendès 34090 Montpellier FRANCE
PROJECT MANAGER: ACOBIOM
+33 (0) 644 890 438
July 2016
Grant writing assistant: identification of heart failure molecular biomarkers (FEDER)
PROJECT MANAGER: Freelance
[email protected]
2015-2016
Website design & creation: • Haras des 3 Mas • Isabelle Devambez - Saddle Fitting PROJECT MANAGER: Institute of Clinical research (Montpellier)
fr.linkedin.com/in/albouge
2013–2015
Team Genetic of rare diseases Project: Splicing analysis of Duchenne Muscular Dystrophy mRNA
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Development of a new method based on Long-Range PCR and long-read RNAseq technologies Identification of DMD splicing regulators by RNAi screening Management : 1 master’s student Valorisation : 1 publication PROJECT MANAGER: Institute of Human Genetics
2012–2013
Team mRNA regulation and development (CNRS-Montpellier-France) Project management : Role of RNAi pathways in Occulopharyngeal Muscular Dystrophy. PROJECT MANAGER: Institute of Functional Genomics (Montpellier)
2010-2012
Team: Drosophila normal and pathological neurobiology Project: DiaTrAl (INSERM/Sanofi) Alzheimer’s disease Diagnostic and Treatment Projects : 1-Determination of Tau toxic roles during mitosis. 2-Identification of new suppressors of tau-induced neurotoxicity. Management : 2 master’s students Valorisation : 2 publications TEACHING ASSISTANT: University of Montpellier • •
2011-2012
Developmental biology (TP-Licence1) Structural and functional biochemistry (TD-Licence1)
POSTDOCTORAL FELLOW: Pasteur Institute
2007–2010
Team: Drosophila Genetics and Epigenetics (CNRS-Paris) Project: Involvement of siRNA in heterochromatin formation Collaborative projects: Role of piRNA in epigenetic mechanisms Identification of miRNA regulators by chemical and genetic screens Valorisation : 4 publications, 2 reviews. PhD FELLOW: University of Dijon
2002–2007
Team: Insects development and chemical communication Thesis : Functional characterization of a new ligand of Drosophila integrin Valorisation : 3 publications. TEACHING ASSISTANT: University of Dijon • •
Cellular biology (TP/TD: Licence1-Licence3) Molecular biology (TP/TD-DUT)
2002-2006
Core Competencies Project management Conception, planning and follow up Project and publication writing Experimental design and set up Data analysis Bibliographic and technologic monitoring Communication (written and spoken) Teaching and management Lab management (organisation, stocks handeling)
Molecular biology Classical techniques: extraction, purification , amplification and quantification of nucleic acids Molecular cloning (classic and Gateway system) Transcriptomics: DNA array, RNAseq (Roche and Illumina)
Cellular Biology Cell culture et transfection (human and insect cells) Immunocytochemisty, in situ hybridization Confocal microscopy Chemical and genetic (RNAi) screens
Bioinformatics Bash, perl, R NGS data analysis Web development: HTML5, CSS3, PHP5 Database: mySQL
English German
Web sites
Education UNIVERSITY OF BURGUNDY: -PhD in Biochemistry, Cellular and Molecular Biology with honours -Master’s degree in Biology with honours
2007 2002
ADDITIONAL COURSE : UCSF: Design and interpretation of clinical trials (coursera – MOOC) OpenClasssrooms: Website creation (html5, CSS3, Bootstrap,WP…) Cnam: Management « Du manager au leader 2.0 » (MOOC)
2016 2015-2016 2015
Publications Bougé AL, Murauer E, Beyne E, Miro J, Varilh J, Taulan M, Koenig M, Claustres M, Tuffery-Giraud S. Targeted RNA-Seq profiling of splicing pattern in the DMD gene: exons are mostly constitutively spliced in human skeletal muscle. Sci Rep. 2017 Jan 3;7:39094. Anne-Laure Bougé and Marie-Laure Parmentier. Tau excess impairs mitosis and kinesin-5 function, leading to aneuploidy and cell death. Dis Model Mech. 2016 Mar 1;9(3):307-19. Carré C, Jacquier C, Bougé AL, de Chaumont F, Besnard-Guerin C, Thomassin H, Pidoux J, Da Silva B, Chalatsi E, Zahra S, Olivo-Marin JC, Munier-Lehmann H, Antoniewski C. AutomiG, a biosensor to detect alterations in miRNA biogenesis and in small RNA silencing guided by perfect target complementarity. PLoS One. 2013 Sep 3;8(9):e74296. de Vanssay A, Bougé AL, Boivin A, Hermant C, Teysset L, Delmarre V, Ronsseray S, Antoniewski C. Profiles of piRNA abundances at emerging or established piRNA loci are determined by local DNA sequences. RNA Biol. 2013 Aug 1;10(8):1233-9.
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de Vanssay A, Bougé AL, Boivin A, Hermant C, Teysset L, Delmarre V, Antoniewski C, Ronsseray S. Paramutation in Drosophila linked to emergence of a piRNA-producing locus. Nature. 2012 Oct 4;490(7418):112-5.
Syed ZA, Bougé AL, Byri S, Chavoshi TM, Tång E, Bouhin H, van Dijk-Härd IF, Uv A. A luminal glycoprotein drives dose-dependent diameter expansion of the Drosophila melanogaster hindgut tube. PLoS Genet. 2012 Aug;8(8):e1002850. Talmat-Amar Y, Arribat Y, Redt-Clouet C, Feuillette S, Bougé AL, Lecourtois M, Parmentier ML. Important neuronal toxicity of microtubule-bound Tau in vivo in Drosophila. Hum Mol Genet. 2011 Oct 1;20(19):3738-45.
References
Fraichard S*, Bouge AL*, Kenerdall T, Chauvel I, Bouhin H and Bunch TA. Tenectin is a novel aPS2bPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila. Dev. Biol. 2010 Apr 15;340(2):504-17. *first co authors
Marie-Laure Parmentier DR2 INSERM IGF Montpellier
[email protected]
Fagegaltier D*, Bouge AL*, Berry B, Poisot E, Sismeiro O, Coppee JY, Théodore L and Antoniewski C. The endogenous siRNA pathway is involved in heterochromatin formation in Drosophila. PNAS. 2009 Dec 15;106(50):21258-63.*first co authors Fraichard S, Bouge AL, Chauvel I and Bouhin H. Tenectin a novel extracellular matrix protein expressed during Drosophila melanogaster embryonic development. Gene Expression Patterns 2006 Oct;6(8):772-6.
Thérèse Commes PU University of Montpellier IRMB Montpellier
[email protected]
Reviews: de Vanssay A, Bougé AL, Boivin A, Hermant C, Teysset L, Delmarre V, Antoniewski C, Ronsseray S. [Inheritance of paramutation in plants and Drosophila]. Med Sci (Paris). 2013 Feb;29(2):136-8. piRNAs and epigenetic conversion in Drosophila. de Vanssay A, Bougé AL, Boivin A, Hermant C, Teysset L, Delmarre V, Antoniewski C, Ronsseray S. Fly (Austin). 2013 Oct 2;7(4).
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