p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis p53_Danio_rerio p53_Barbus p53_Xiph_maculatus p53_Xiph_hellerii p53_Tetraodon_miurus p53_Platichthys_flesus p53_Ictalurus p53_rainbow_trout

-MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPS----QAMDDLML -MEEPQSDPSIEPPLSQETFSDLWKLLPENNVLSPLPS----QAVDDLML -MEEPQSDPSIEPPLSQETFSDLWKLLPENHVLSPLPS----QAVDDLML -MEEPQSDPSIEPPLSQETFSDLWKLLPENNVLSPLPS----QAVDDLML -MEEPQSDPSIEPPLSQETFSDLWKLLPENNVLSPLPS----QAVDDLML -MEEPHSDLSIEPPLSQETFSDLWKLLPENNVLSDSLS----PPMDHLLL -MEESQSDLSLEPPLSQETFSDLWKLLPENNLLTTSLN----PPVDD-LL -MEEPQSDLSIELPLSQETFSDLWKLLPPNNVLSTLP--SS-DSIEELFL -MEEPQSDLSIELPLSQETFSDLWKLLPPNNVLSTLP--SS-DSIEELFL -MEEPQSDLSIEPPLSQETFSDLWKLLPPKNLLS-----AL-EPMEDLLL -MEDSQSDMSIELPLSQETFSCLWKLLPPDDILPTTATGSP-NSMEDLFL -MEEAQSDLSIEPPLSQETFSDLWNLLPENNVLSPVLS----PPMDDLLL -MEEQQSDLSIEPPLSQETFSDLWKLLPQNNVLSTPL--SP-NSMEDLLL -MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPS----QAMDDLML -MEESQSDISLELPLSQETFSGLWKLLPPEDILP-----SP-HCMDDLLL -MEESQAELNVEPPLSQETFSDLWNLLPENNLLSSELS----APVDDLLP -MEESQAELNVEPPLSQETFSDLWNLLPENNLLSSELS----APVDDLLP -MEESQAELGVEPPLSQETFSDLWNLLPENNLLSSELS----APVDDLLP -MEESQSELGVEPPLSQETFSDLWKLLPENNLLSSELS---LAAVNDLLL -MEESQSELNIDPPLSQETFSELWNLLPENNVLSSELC----PAVDELLL -MEESQAELGVEPPLSQETFSDLWKLLPENNLLSSELS----PAVDDLLL -MQEPPLELTIEPPLSQETFSELWNLLPENNVLSSELS----SAMNELPL -MAEEMEPL-LEP---TEVFMDLWSMLP--------------YSMQQLPL --MEPSSETGMEPPLSQETFEDLWSLLP--------------DPLQTGTG --MEPSSETGMDPPLSQETFEDLWSLLP--------------DPLQTVTC ----------MAQN-DSQEFAELWEK---NLIIQPPGGGSCWDIINDE-----------MAE---SQEFAELWER---NLISTQEAG-TCWELIND---MEEADLT----LPLSQDTFHDLWNN-----VFLSTENESLP--------MEEADLT----LPLSQDTFHDLWNN-----VFLSTENESLA--------MEEENIS----LPLSQDTFQDLWDN-----VSAPPISTIQTAALENEAW -MDEQGLDGMQILPGSQDSFSELWAS-----VQTPSIATI---------MEGNGERDTMMVEPPDSQEFAELWLR---NLIVR---DNSLWGKEEE-----MADLAENVSLPLSQESFEDLWKMNL-NLVAVQPPETESWVGYDNFMM

45 45 45 45 45 45 44 46 46 43 48 45 46 45 43 45 45 45 46 45 45 45 31 34 34 34 30 32 32 40 34 41 46

p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis p53_Danio_rerio p53_Barbus p53_Xiph_maculatus p53_Xiph_hellerii p53_Tetraodon_miurus p53_Platichthys_flesus

SPDDIEQWFTED-PGPDEAPRMPE------AAPRVAPAPAAPTPAAPAPA SPDDLAQWLTED-PGPDEAPRMSE------AAPHMAPTPAAPTPAAPAPA SPDDLAQWLTED-PGPDEAPRMSE------AAPPMAPTPAAPTPAAPAPA SPDDLAQWLTED-PGPDEAPRMSE------AAPPMAPTPAAPTPAAPAPA SPDDLAQWLTED-PGPDEAPRMSE------AAPPMAPTPAAPTPAAPAPA SPEEVASWLGE---NPDGDGHVSA------APVSEAPTSAGPALVAPAPA SAEDVANWLNE---DPEEGLRVPA------APAPEAPAPAAPALAAPAPA S-ENVAGWLED----PGEALQGSAAAAAPAAPAAEDPVAETPAPVASAPA S-ENVTGWLED----SGGALQG---VAAAAASTAEDPVTETPAPVASAPA P-QDVTSWLGD----ADEALPVCT------APAEG-PAPEAPAPAAPAPP P-QDVAELLEG----PEEALQVS-------APAAQEPGTEAPAPVAPASA SSEDVENWFDK---GPDEALQMSA------APAPKAPTPAASTLAAPSPA SPEDVANWLDD----PDEALQV------PAAAITGDPVTETSAPVAPPPA SPDDIEQWFTED-PGPDEAPRMPE------AAPPVAPAPAAPTPAAPAPA P-QDVEEFFEG----PSEALRVSG------APAAQDPVTETPGPVAPAPA Y-TDVATWLDE---CPNEAPQMPE------PSA-----PAAPPPATPAPA Y-TDVATWLDE---CPNEAPQMPE------PSA-----PAAPPPATPAPA YSEDVVTWLDE---CPNEAPQMPE------P----------PAQAALAPA SP--VTNWLDE---NPDDASRVPA------PPA-----ATAPAPAAPAPA P-ESVVNWLDE---DSDDAPRMPA------TSA-----PTAPGPAP---SPEDVANWLDE---RPDEAPQMPE------PPA-----PAAPTPAAPAPA S-EDVANWLDE---APDDASGMSA------VPA-----PAAPAPATPAPA P-EDHSNWQELSPLEPSDPPPPP-------PPPPLPLAAAAPPPLNPPTP QMENFAEFSEYP-LAPDMTVLQEG---------------------LMGNT RLDNLSEFPDYP-LAADMTVLQEG---------------------LMGNA -EYLPGSFDPN---FFENVLEE------------------QPQPST----EYLPSSFDPN---IFDNVLTE------------------QPQPST---PPEGLLSQNMD---FWED-----------------------PETMQE--T PPEGLLSQNMD---FWED-----------------------PETMQE--T PAERQMNMMCN---FMDSTFNEALF-------------NLLPEPPSR--D -AEEFDDHLGN---LLQNGFDMNLF-------------ELPPEMVAK--D

88 88 88 88 88 86 85 91 88 81 86 86 86 88 82 80 80 76 80 76 81 80 73 62 62 58 54 54 54 72 65

p53_Ictalurus p53_rainbow_trout

---IPDDLQEV---PCDVLLSDML----------------QPQSSS---- 65 EAPLQVEFDPS---LFEVSATEP-----------------APQPSISTLD 76

p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis p53_Danio_rerio p53_Barbus p53_Xiph_maculatus p53_Xiph_hellerii p53_Tetraodon_miurus p53_Platichthys_flesus p53_Ictalurus p53_rainbow_trout

PSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLA PSWPLSSSVPSQKTYHGSYGFRLGFLHSGTAKSVTCTYSPDLNKMFCQLA PSWPLSSSVPSQKTYHGSYGFRLGFLHSGTAKSVTCTYSPDLNKMFCQLA PSWPLSSSVPSQKTYHGSYGFRLGFLHSGTAKSVTCTYSPDLNKMFCQLA PSWPLSSSVPSQKTYHGSYGFRLGFLHSGTAKSVTCTYSPDLNKMFCQLA TSWPLSSSVPSHKPYRGSYGFEVHFLKSGTAKSVTCTYSPGLNKLFCQLA TSWPLSSSVPSQKTYHGNYGFRLGFLHSGTAKSVTCTYSPCLNKLFCQLA TPWPLSSSVPSYKTYQGDYGFRLGFLHSGTAKSVTCTYSPSLNKLFCQLA TPWPLSSSVPSYKTFQGDYGFRLGFLHSGTAKSVTCTYSPSLNKLFCQLA ASWPLSSFVPSHKTFQGNYGFRLGFLQSGTAKSVTCTYSPSLNKLFCQLA TPWPLSSSVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSISLNKLFCQLA TSWPLSSSVPSQNTYPGVYGFRLGFLHSGTAKSVTCTYSPSLNKLFCQLA TPWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPPLNKLFCQLA PSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPDLNKLFCQLA TPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLA TSWPLSSFVPSQKTYPGNYGFRLGFLQSGTAKSVTCTYSPSLNKLFCQLA TSWPLSSFVPSQKTYPGNYGFRLGFLQSGTAKSVTCTYSPSLNKLFCQLA TSWPLSSFVPSQKTYPGNYGFRLGFLHSGTAKSVTCTYSPSLNKLFCQLA TSWPLSSFVPSQKTYPGSYDFRLGFLHSGTAKSVTCTYSPALNKLFCQLA -SWPLSSSVPSPKTYPGTYGFRLGFLHSGTAKSVTWTYSPLLNKLFCQLA TSWPLSSFVPSQKTYPGSYGFHLGFLHSGTAKSVTCTYSPALNKLFCQLA ISWPLSSFVPSQKTYPGAYGFHLGFLQSGTAKSVTCTYSPPLNKLFCQLA PRAAPSPVVPSTEDYGGDFDFRVGFVEAGTAKSVTCTYSPVLNKVYCRLA VPTVTSSAVPSTEDYAGSYGLKLEFQQNGTAKSVTCTYSTDLNKLFCQLA VPTVTSCAVPSTDDYAGKYGLQLDFQQNGTAKSVTCTYSPELNKLFCQLA --LPPTSTVPETSDYPGDHGFRLRFPQSGTAKSVTCTYSPDLNKLFCQLA --SPPTASVPVATDYPGEHGFKLGFPQSGTAKSVTCTYSSDLNKLFCQLA KNVPTAPTVPAISNYAGEHGFNLEFNDSGTAKSVTSTYSVKLGKLFCQLA KNVPTAPTVPAISNYAGEHGFNLEFNDSGTAKSVTSTYSVKLGKLFCQLA GANSSSPTVPVTTDYPGEYGFKLRFQKSGTAKSVTSTYSEILNKLYCQLA SVTPPSSTVPVVTDYPGEYGFQLRFQKSGTAKSVTSTFSELLKKLYCQLA --SPPTSTVPVTSDYPGLLNFTLHFQESSGTKSVTCTYSPDLNKLFCQLA TGSPPTSTVPTTSDYPGALGFQLRFLQSSTAKSVTCTYSPDLNKLFCQLA

138 138 138 138 138 136 135 141 138 131 136 136 136 138 132 130 130 126 130 125 131 130 123 112 112 106 102 104 104 122 115 113 126

p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis p53_Danio_rerio p53_Barbus p53_Xiph_maculatus p53_Xiph_hellerii

KTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSD---KTCPVQLWVDSTPPPGSRVRAMAIYKQSQHMTEVVRRCPHHERCSD---KTCPVQLWVDSTPPPGSRVRAMAIYKQSQHMTEVVRRCPHHERCSD---KTCPVQLWVDSTPPPGSRVRAMAIYKQSQHMTEVVRRCPHHERCSD---KTCPVQLWVDSTPPPGSRVRAMAIYKQSQHMTEVVRRCPHHERCSD---KTCPVQVWVESPPPPGTRVRALAIYKKSQHMTEVVRRCPHHERCSD---KTCPVQLWVDSTPPPGTRVRAMAIYKKSQHMTEVVRRCPHHERCSD---KTCPVQLWVSSTPPPGTRVRAMAIYKKLQYMTEVVRRCPHHERSSE---KTCPVQLWVNSTPPPGTRVRAMAIYKKLQYMTEVVRRCPHHERSSE---KTCPVQLWVSSAPPPGTRVRAMAIYKNSQHMTEVVRRCPHHERCSENEAS KTCPVQLWVTSTPPPGTRVRAMAIYKKSQHMTEVVRRCPHHERCSD---KTCPVQLWVDSTPPPGTRVRAMAIYKKSQHMTEVVRRCPHHERCSD---KTCPVQLWVDSTPPPGTRVRAMAIYKKSQHMTEVVKRCPHHERCSD---KTCPVQLWVDSAPPPGTRVRAMAIYKQSQYVTEVVRRCPHHERCSD---KTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRC--HHRCS----KTCPVQLWVDSPPPPGTRVRAMAIYKKLEHMTEVVRRCPHHERSSDY--KTCPVQLWVDSPPPPGTRVRAMAIYKKLEHMTEVVRRCPHHERSSDY--KTCPVQLWVDSPPPPGTRVRAMAIYKKLEHMTEVVRRSPHHERSSDY--KTCPVQLWVSSPPPPGTRVRAMAIYKKSEYMTEVVRRCPHHERSSDY--KTCPVQLWVSSPPPPNTCVRAMAIYKKSEFVTEVVRRCPHHERCSDS--KTCPVQLWVSSPPPPGTRVRAMAIYKKSEYMTEVVRRCPHHERCSDY--KTCPVQLWVRSPPPPGTCVRAMAIYKKSEFMTEVVRRCPHHERCPDS--KPCPVQVRVGVAPPPGSSLRAVAVYKKSEHVAEVVRRCPHHERCGGG--KTCPLLVRVERPPPLGSILRATAVYKKSEHVAEVVKRCPHHERSVEP--KTCPLLVRVESPPPRGSILRATAVYKKSEHVAEVVKRCPHHERSVEP--KTCPVQMVVDVAPPQGSVVRATAIYKKSEHVAEVVRRCPHHERTPD---KTCPVQMVVNVAPPQGSVIRATAIYKKSEHVAEVVRRCPHHERTPD---KTTPIGVLVKEEPPQGAVIRATAVYKKTEHVGEVVKRCPHHQ-------KTTPIGVLVKEEPPQGAVIRATSVYKKTEHVGEVVKRCPHHQ--------

184 184 184 184 184 182 181 187 184 181 182 182 182 184 175 177 177 173 177 172 178 177 170 159 159 152 148 146 146

p53_Tetraodon_miurus p53_Platichthys_flesus p53_Ictalurus p53_rainbow_trout

KTSLVEVLLGKDPPMGAVLRATAIYKKTEHVAEVVRRCPHHQ-------KTSPVEVLLSKEPPQGAVLRATAVYKKTEHVADVVRRCPHHQ-------KTCPVLMAVSSSPPPGSVLRATAVYKRSEHVAEVVRRCPHHERSND---KTCPVQIVVDHPPPPGAVVRALAIYKKLSDVADVVRRCPHHQSTSE----

164 157 159 172

p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis p53_Danio_rerio p53_Barbus p53_Xiph_maculatus p53_Xiph_hellerii p53_Tetraodon_miurus p53_Platichthys_flesus p53_Ictalurus p53_rainbow_trout

-SDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLRVEYSDDRNTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLRVEYSDDRNTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLRVEYSDDRNTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLRVEYSDDRNTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLHAEYVDDRTTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYEPPEVGSDCTTIH -GDGLAPPQHLIRVEGNMHAEYLDDKQTFRHSVVVPYEPPEVGSDCTTIH -GDSLAPPQHLIRVEGNLHAEYLDDKQTFRHSVVVPYEPPEVGSDCTTIH DPRGRAPPQHLIRVEGNLHAEYVDDRQTFRHSVLVPYESPEVGSDCTTIH -GDGLAPPQHLIRVEGNPYAEYLDDRQTFRHSVVVPYEPPEVGSDYTTIH -SDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYEPPEVGSECTTIH -SDGLAPPQHLIRVEGNLRAEYLDDKHTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLHAEYSDDRNTFRHSVVVPYEPPEVGSDCTTIH -GDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIH -SDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYESPEIDSECTTIH -SDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYESPEIDSECTTIH -SDGLAPPQHLIRVEGNLRAEYFDDRNTFRHSVVVPYESPEIESECTTIH -SDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLRAKYLDDRNTFRHSVVVPYEPPEVGSDYTTIH -SDGLAPPQHLIRVEGNLRAEYLDDRNTFRHSVVVPYEPPEVGSDCTTIH -SDGLAPPQHLIRVEGNLHAKYLDDRNTFRHSVVVPYEPPEVGSDCTTIH -TDGLAPAQHLIRVEGNPQARYHDDETTKRHSVVVPYEPPEVGSDCTTVL -GDDPAPPSHLMRVEGNSKAYYMEDVGTGRHSVCVPYEGPQVGTECTTVL -GEDAAPPSHLMRVEGNLQAYYMEDVNSGRHSVCVPYEGPQVGTECTTVL GDNLAPAGHLIRVEGNQRANYREDNITLRHSVFVPYEAPQLGAEWTTVL GDGLAPAAHLIRVEGNSRALYREDDVNSRHSVVVPYEVPQLGSEFTTVL SEDLSDNKSHLIRVEGSQLAQYFEDPNTRRHSVTVPYERPQLGSEMTTIL SEDLSDNKSHLIRVEGSQLAQYFEDPNTRRHSVTVPYERPQLGSEMTTIL NEDSAEHRSHLIRMEGSERAQYFEHPHTKRQSVTVPYEPPQLGSEFTTIL TEDTAEHRSHLIRLEGSQRALYFEDPHTKRQSVTVPYEPPQLGSETTAIL SSDGPAPPGHLLRVEGNSRAVYQEDGNTQAHSVVVPYEPPQVGSQSTTVL NNEGPAPRGHLVRVEGNQRSEYMEDGNTLRHSVLVPYEPPQVGSECTTVL

233 233 233 233 233 231 230 236 233 231 231 231 231 233 224 226 226 222 226 221 227 226 219 208 208 201 197 196 196 214 207 209 222

p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis p53_Danio_rerio p53_Barbus

YNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGKLLGRDSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDPSGNLLGRNSFEVRICACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDPSGNLLGRNSFEVRICACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDPSGNLLGRNSFEVRVCACPGRDRR YKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEGSSGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGKLLGRNSFEVRICACPGRDRR YKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRR YNFMCNSSCMGGMNRRPILTIITLEDSCGNLLGRNSFEVRVCACPGRDRR YNFMCNSSCMGGMNRRPILTIITLEDSCGNLLGRNSFEVRVCACPGRDRR YNFMCNSSCMGGMNRRPILTIITLEDSRGNLLGRSSFEVRVCACPGRDRR YNFMCNSSCMGGMNRRPILTIITLEDASGNLLGRNSFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLEDSSGNVLGRNSFEVRVCACPGRDRR YNFMCNSSCMGGMNRRPILTIITLEDSNGNLLGRNSFEVRVCACPGRDRR YNFMCNSSCMGGMNRRPIITIITLEDSNGKLLGRNSFEVRVCACPGRDRR YNFMCNSSCMGGMNRRPILTILTLEGPGGQLLGRRCFEVRVCACPGRDRK YNYMCNSSCMGGMNRRPILTIITLESPEGLLLGRRCFEVRVCACPGRDRR YNYMCNSSCMGGMNRRPILTIITLETPEGLLLGRRCFEVRVCACPGRDRR LNYMCNSSCMGGMNRRPILTIITLETQEGQLLGRRSFEVRVCACPGRDRK YNFMCNSSCMGGMNRRPILTIISLETHDGQLLGRRSFEVRVCACPGRDRK

283 283 283 283 283 281 280 286 283 281 281 281 281 283 274 276 276 272 276 271 277 276 269 258 258 251 247

p53_Xiph_maculatus p53_Xiph_hellerii p53_Tetraodon_miurus p53_Platichthys_flesus p53_Ictalurus p53_rainbow_trout

LSFMCNSSCMGGMNRRPILTILTLETTEGEVLGRRCFEVRVCACPGRDRK LSFMCNSSCMGGMNRRPILTILTLETTEGEVLGRRCFEVRVCACPGRDRK LSFMCNSSCMGGMNRRPILTILTLETQEGIVLGRRCFEVRVCACPGRDRK LSFMCNSSCMGGMNRRQILTILTLETPDGLVLGRRCFEVRVCACPGRDRK YNYMCNSSCMGGMNRRPILTIITLETQDGHLLGRRTFEVRVCACPGRDRK YNFMCNSSCMGGMNRRPILTIITLETQEGQLLGRRSFEVRVCACPGRDRK

246 246 264 257 259 272

p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis p53_Danio_rerio p53_Barbus p53_Xiph_maculatus p53_Xiph_hellerii p53_Tetraodon_miurus p53_Platichthys_flesus p53_Ictalurus p53_rainbow_trout

TEEENLRKKGEP---HHELPPGSTKRALPNNTS-SSPQPKKKP------TEEENFRKKGEP---CHELPPGSTKRALPNNTS-SSPQPKKKP------TEEENFRKKGEP---CHQLPPGSTKRALPNNTS-SSPQPKKKP------TEEENFRKKGEP---CHQLPPGSTKRALPNNTS-SSPQPKKKP------TEEENFRKKGEP---CHQLPPGSTKRALPNNTS-SSPQPKKKP------TEEENFRKKGGL---CPEPTPGNIKRALPTSTS-SSPQPKKKP------TEEENFRKKGEP---CPELPPGSSKRALPTTTTDSSPQTKKKP------TEEKNFQKKGEP---CPELPPKSAKRALPTNTS-SSPQPKRKT------TEEKNFQKKGEP---CPELPPKSAKRALPTNTS-SSPPPKKKT------TEEENLRKKQ-R---CPELPQGSAKRALPTNTS-SSPQSKRKP------TEEENFRKKEEH---CPELPPGSAKRALPTSTS-SSPQQKKKP------TEEENFRKRGEP---CPEPPPRSTKRALPNGTS-SSPQPKKKP------TEEENFRKKGEL---CPELPPGSTKRALPTGTS-SSPQPKKKP------TEEENFRKKGES---CPKLPTGSIKRALPTGSS-SSPQPKKKP------TEEENFRKKEVL---CPELPPGSAKRALPTCTS-ASPPQKKKP------TEEENLRKKGQS---CPEPPPRSTKRALPTNTS-SSPQPKKKP------TEEENLRKKGQS---CPEPPPRSTKRALPTNTS-SSPQPKKKP------TEEENFRKKGQS---CPEPPPGSTKRALPSSTS-SSPQQKKKP------TEEENFLKKGQS---CPEPPPGSTKRALPTSTS-SSPVQKKKP------TEEENFHKKGEP---CPEPPPGSTKRALPPSTS-SSPPQKKKP------TEEENFHKKGQS---CPELPTGSAKRALPTGTS-SSPPQKKKP------TEEENFRKKGEP---CPEPPPGSTKRALPPSTS-STPPQKKKP------IEEENFRKRG--------GAGGVAKRAMSPPTEAPEPPKKRVLN-----TEEDNCTKKRGL---KP-----NGKRELSHPPSSDPPLPKKRLV-----E TEEDNYTKKRGL---KP-----SGKRELAHPPSSEPPLPKKRLV-----V TEESNFKKDQET--KTMAKTTTGTKRSLVKESSSATLRPEGSKKAKGS-TEESNFRKDQET--KTLDKIPSANKRSLTKDSTSSVPRPEGSKKAKLSGTEEGNL-------EKS--GTKQTKKRKSAPA---PDTSTAKKSKSASSGE TEEGNL-------EKS--GTKQTKKRKSAPA---PDTSTAKKSKSASSGE TEETNS-------TKMQNDAKDAKKRKSVPT---PDSTTIKKSKTASSAE TDEESS-------TKTPNGPKQTKKRKQAPSNSAPHTTTVMKSKSSSSAE TEESNFKKQQEP--KTSGKTLT--KRSMKDPPS----HPEASKKSKNS-TEEINLKKQQETTLETKTKPAQGIKRAMK-EASLPAPQPGASKKTKSSPA

322 322 322 322 322 320 320 325 322 319 320 320 320 322 313 315 315 311 315 310 316 315 305 295 295 297 294 284 284 304 300 299 321

p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis

-LDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLK-LDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPAGSRAHSSHLK-LDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPAGSRAHSSHLK-LDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPAGSRAHSSHLK-LDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPAGSRAHSSHLK-LDAEYFTLKIRGRKNFEILREINEALEFKDAQTEKEPGESRPHSSYPK-LDGEYFILKIRGRERFEMFRELNEALELKDAQAEKEPGGSRAHSSYLK-LDGEYFTLKIRGQERFKMFQELNEALELKDAQALKASEDSGAHSSYLK-LDGEYFTLKIRGHERFKMFQELNEALELKDAQASKGSEDNGAHSSYLK-ADGEYFTLKIRGRKRFEVFRELNEALELKDAQAAGESGDGRAQASCLK-LDGEYFTLKIRGRERFEMFRELNEALELKDARAAEESGDSRAHSSYPK-LDGEYFTLKIRGRARFEMFQELNEALELKDAQAEKEPGESRPHPSYLK-LDGEYFTLKIRGRERFEMFRELNEALELKDTQAEKDSGESRAHSSYLK-LDEEYFTLQIRGRERFEMLREINEALELKDAMAGKESAGSRAHSSHLK-LDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLK-LDGEYFTLQIRGFKRYEMFRELNDALELKDALDGREPGESRAHSSHLK-LDGEYFTLQIRGFKRYEMFRELNDALELKDALDGREPGESRAHSSHLK-LDGEYFTLQIRGRKRFEMFRELNEALELMDAQAGREPGESRAHSSHLK-LDGEYFTLQIRGRERFEMFRELNDALELKDAQTARESGENRAHSSHLK-LDGEYFTLQIRGRERYEMFRNLNEALELKDAQSGKEPGGSRAHSSHLK-LDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGESRAHSSHLK-LDGEYFTLQIRGRERFEMFRELNEALELKDAQSGKEPGGSRAHSSHLK-PDNEIFYLQVRGRRRYEMLKEINEALQLAEGGSAPRPSKGR----RVKEDDEETFTLLIKGRSRYEMIKKLNDALELQESLDQQ-----KLSIKCRKDDDEEIFTLRIKGRSRYEMIKKLNDALELQESLDQQ-----KVTIKCRK-

370 370 370 370 370 368 368 373 370 367 368 368 368 370 361 363 363 359 363 358 364 363 349 339 339

p53_Danio_rerio p53_Barbus p53_Xiph_maculatus p53_Xiph_hellerii p53_Tetraodon_miurus p53_Platichthys_flesus p53_Ictalurus p53_rainbow_trout

SSDEEIFTLQVRGRERYEILKKLNDSLELSDVVPASDAEKYRQKFMTK-SSDEEIYTLQVRGKERYEMLKKINDSLELSDVVPPSEMDRYRQKLLTK-DEDKEIYTLSIRGRNRYLWFKSLNDGLE----------LMDKTGPKIK-DEDKEIYTLSIRGRNRYLWFKSLNDGLE----------LMDKTGPKIK-EDNNEVYTLQIRGRKRYEMLKKINDGLD-------LLENKPKSKATHR-EEDKEVFTVLVKGRERYEIIKKINEAFEG-----AAEKEKAKNKVAVK-SSDDEIYTLQVRGKERYEFLKKINDGLELSDVVPPADQEKYRQKLLSKTC VSDDEIYTLQIRGKEKYEMLKKFNDSLELSELVPVADADKYRQKCLTKRV

p53_Homo_Sapiens p53_Afr_Green_Monk. p53_Mac_Fascicularis p53_Mac_Fuscata p53_Mac__Mulatta p53_Guinea_pig p53_Bugs_Bunny p53_Gold_hamster p53_Chinese_hamster p53_Gerbil p53_Rat p53_Marmota_monax p53_Spalax p53_Tree_shrew p53_Mus_musculus p53_Bos_indicus p53_Bos_taurus p53_Sheep p53_Pig p53_Canis_fam p53_Beluga_Whale p53_Felix_Catus p53_Galus p53_X_Tropicalis p53_X_laevis p53_Danio_rerio p53_Barbus p53_Xiph_maculatus p53_Xiph_hellerii p53_Tetraodon_miurus p53_Platichthys_flesus p53_Ictalurus p53_rainbow_trout

----SKKGQSTSRHKKLMFKTE-GPDSD 393 ----SKKGQSTSRHKKFMFKTE-GPDSD 393 ----SKKGQSTSRHKKFMFKTE-GPDSD 393 ----SKKGQSTSRHKKFMFKTE-GPDSD 393 ----SKKGQSTSRHKKFMFKTE-GPDSD 393 ----SKKGQSTSCHKKLMFKRE-GLDSD 391 ----AKKGQSTSRHKKPMFKRE-GPDSD 391 ----SKKGQSASRLKKLMIKRE-GPDSD 396 ----SKKGQSASRLKKLMIKRE-GPDSD 393 ----TKKDKSTSPRKNPMIKRE-EPDSD 390 ----TKKGQSTSRHKKPMIKKV-GPDSD 391 ----SKKGQSTSRHKKIIFKRE-GPDSD 391 ----SKKGQSTSRHKKLMIKRE-GPDSD 391 ----SKKGQSTSRHRKLMFKTE-GPDSD 393 ----TKKGQSTSRHKKTMVKKV-GPDSD 384 ----SKKRPSPSCHKKPMLKRE-GPDSD 386 ----SKKRPSPSCHKKPMLKRE-GPDSD 386 ----SKKGPSPSCHKKPMLKRE-GPDSD 382 ----SKKGQSPSRHKKPMFKRE-GPDSD 386 ----AKKGQSTSRHKKLMFKRE-GLDSD 381 ----SKKGQSPSRHKKLMFKRE-GPDSD 387 ----AKKGQSTSRHKKPMLKRE-GLDSD 386 ----VE-GPQPSCGKKLLQK-----GSD367 ----CRDEIKPKKGKKLLVKDE-LQDSE 362 ----CRDEIKPKKGKKLLVKDE-QPDSE 362 NKKENRESSEPKQGKKLMVKDEGRSDSD 373 GKKKDGQTPEPKRGKKLMVKDE-KSDSD 369 -----QEIPAPSSGKRLLKGG---SDSD 342 -----QEIPAPSSGKRLLKGG---SDSD 342 -----PDGPIPPSGKRLLHRGE-KSDSD 367 -----QELPVPSSGKRLVQRGE-RSDSD 365 RKERDGAAGEPKRGKKRLVKEE-KCDSD 376 AKRDFGVG--PKKRKKLLVKEE-KSDSD 396

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