Figure 1.1: Scheme of a longitudinal section through the pistil with all parts of the plant female reproductive organs and principle of the self-incompatibility response: non-self pollen (right) germinates and forms a pollen tube. Self-pollen (left) germination is inhibited and the pollen rejected.
Plant enhancer GAL4
UAS
DNA
GAL4 GFP
Figure 2.1: Principal of the Enhancer-Trap strategy, that was used for the establishment of a collection of A. thaliana lines in the laboratory of Jim Haseloff. A transgene (yellow bar) was randomly inserted into the plant genome. The transgene contains the sequence for the yeast transcription factor GAL4, as well as the GFP gene whose transcription is controlled by GAL4 recognition sequences (UAS ). When the transgene is inserted close to an enhaner, GAL4 is expressed and activates GFP expression. This collection of plants is a very useful tool to identify plant enhancers that are active in a specific tissue. In the case of the N9292 line, GFP has a reticulum tag and it is highly expressed in papillar cells, revealing that the transgene is probably inserted in proximity of a stigmatic enhancer. According to http://www.plantsci.cam.ac.uk/Haseloff/
Construction pSLR1 AlSNX1
GFP6
t
Description
Destination vector
Selection in planta
Plant
Objective
Endosomal protein Sorting Nexin 1 - Green Fluorescent Protein fusion
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