photochemical internalization, PCI. Then, using Giant unilamellar vesicles, we asymmetrically oxidize the membranes. We observed different shape transitions, ...
Membrane permeabilization and shape transition: role of asymetry and depth of modification Stéphanie Bonneau1, Julien Heuvingh2, Halina Mojzisova1, Daniel Brault1. 1 2
ANBioPhi - Université Paris 6 - CNRS FRE3207 - France PMMH - Université Paris 7 - ESPCI - CNRS UMR7636 - France
The photosensitizing properties of three chlorins are compared in solution and when incorporated in dioleoyl-phosphatidylcholine vesicules. In solution, they possess a similar efficacy to generate singlet oxygen and a similar ability to induce the peroxidation. However, the role of the photosensitizer localization within the lipidic bilayer is strongly highlighted, when chlorins are incorporated in liposomes, both by the changes in order of peroxidation efficacy but by the measurements of the photoinduced permeation of the liposomes. The results are discussed in relation with the technology of photochemical internalization, PCI. Then, using Giant unilamellar vesicles, we asymmetrically oxidize the membranes. We observed different shape transitions, such as budding, typical of membrane curvature modifications. The asymmetry of the shape transitions are in accordance to a lowered effective spontaneous curvature of the leaflet targeted. This effect is interpreted as a decreased preferred area of the targeted leaflet compared to the other, due to the secondary products of oxidation. Permeabilization of GUV by photooxidation is interpret as the opening of a pore above a critical membrane tension due to the budding of vesicles. The effective spontaneous curvature of photosensitized vesicle at lysis was estimated. Additionally photooxidation was shown to be fusogenic.
properties, such vesicles are interesting theoretical models of how primitive ... Giant unilamellar vesicles and a chlorin, we asymmetrically photo-damage.
Jan 19, 2009 - The Curve sketching option allows you to draw and refine a curve in the ... You can iterate by drawing as many sketches as you want; the curve ...
Feb 15, 2019 - Ceferino Varón-González1,2, Luisa F. Pallares3, Vincent Debat1â and Nicolas Navarro2,4*â . 1 Institut .... landmarks located on the mid sagittal plan) recorded on each skull ..... patterns of shape variation described by G result
The technique em- ployed was to present the .... this manner rose as a smooth linear function of ... ject matches a rotating circle, viewed in dark space, to a given ...
and representativeness, differences between forms, individual differences, ... stance of a more general class of shape .... The only conclusion which is war-.
Aug 14, 2007 - once the biggest in South ... In response to the twin pressures of Peak Oil and Climate Change, some pioneering ... start of this 21st century are Climate Change and Peak .... Local: this is where local Transition Initiatives play a si
intra-class dispersion matrix and the inter-class dispersion ... aN Юt. Solutions standardization, i.e. /i Р/i ¼ 1, results then in ai ..... These curves are approximated by composite B ezier curves and ... 8 presents the results: expertise in wh
the goals of more accurate diagnoses, better treatments, ... To accurately study inter-subject shape ..... demonstrated to be able to accurately and efficiently.
the vertical masks could be rotated to vary the slant of the sides of the shape response. A more detailed description of this apparatus is given by Kaiser (1966).
Cardiac chambers and major vessels were segmented using shape-constrained de- formable models while the left atrial appendage (LAA) was extracted using ...
the configuration of polar perspective, but the distortions im- plicit in that .... from the edge so that an area of the pattern 28 cm wide and running the width of the ...
Jan 19, 2009 - Students attending this course should be familiar with the CATIA ... More Exercises for Practice ...... Pick: Projects vertices onto selected edge,.
8â10 times through a stack of two polycarbonate membrane. Fig. ... Then, 10 ms later, the resulting ...... [39] P.W. Atkins, Physical Chemistry, 4th ed., Oxford Univ.Missing:
related to the porosity of pit membranes by a process known as air seeding .... may affect membrane porosity, liquids of different surface tension (s) were used to ...
transition control by small roughness elements. Linear and nonlinear ..... Simulations help to answer this question. ... A spanwise periodic array of ... the first three cases only the roughness element height is varied ..... remains a key issue.
B and S . Then it calculates the expected value of the two functions based on B and .... 2 d(Tx,HS (x))·f(Tx) defines the local conditional probability than p(T |S) ...
In order to use a particular physical system for quantum computation, it is necessary ... photon emission from a single rubidium atom trapped at the focal point of.
Abstract: This paper introduces a new approach for the verification of behaviour properties in hybrid systems. By using Petri nets and object oriented concepts ...
Page 1 .... several summary measures but may be unable to rank order all income-generating ..... finally to calculate the appropriate ratios between them. In short ...
