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Mechanical stress increases vocal fold transepithelial potential difference Christopher Atkins, MA Kimberly V. Fisher, Ph.D. Northwestern University Department of Communication Sciences and Disorders Evanston, IL. USA Vocal fold epithelium functions as a physical barrier that defends against numerous biological threats including mechanical stress inherent in voice production, airway protection, and swallowing. It is well established that eukaryotic cells and tissues are mechanosensitive and this sensitivity can be biologically communicated through numerous chemical and molecular responses to external mechanical stressors. We hypothesized that a mechanosensitivity of vocal fold epithelium could be detected by changes in the tissue's bioelectric properties in response to stretch. We further questioned whether vocal fold epithelial sodium channels (ENaC) contribute to any observed mechanosensitivity of this tissue. Fresh ovine vocal fold mucosa (N=20) were mounted in an Ussing Chamber System. The transepithelial potential difference (PD) and shortcircuit current (Isc) were acquired from both a control group (n=10), and an experimental group (n=10), before, during, and after stretch. For both groups, imposition of a hydraulic pressure gradient (5 cm H2O applied to the basal surface) stretched the tissues. In the experimental group,10µM of amiloride, an inhibitor of epithelial sodium channels, was added prior to stretch to determine the role of Na+ transport in supporting mechanosensitivity. Tissues were maintained in a viable state for the duration of the experiment. Stretch increased the PD (p
model parameters describe qualitatively the physiological properties of the vocal folds. The results of the inversion procedure are compared with perturbation ...
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In Defense of the Vocal Fold and Phonation. Kimberly V. Fisher. Northwestern University Department of Communication Sciences and Disorders. Evanston, IL.
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19 juin 2016 - Prix des places : Adultes plein tarif, le jour du concert = 15 € - Réservations tarif préférentiel = 12 € ... Nombre de places à réserver : adultes.