INFLUENCE OF DIFFERENT MICROALGAE ... - Marc Lacuisse
used, but in the long term other factors (such as in biochemical composition of algae, algae cell hardness and physico-chemical parameters) also determine ...
INFLUENCE OF DIFFERENT MICROALGAE SPECIES ON SIZE AND GROWTH OF Brachionus plicatilis Marc A. Lacuisse, Rui J. Rocha, Luis E.C. Conceição, Pavlos Makridis, Maria Teresa Dinis
CCMar
Centre of Marine Sciences, Algarve University, Faro, PORTUGAL
Centre of Marine Sciences
Æ Table 1: Average cell diameter and dry weight of algae strains
Introduction: First feeding of fish larvae with a small mouth opening, in species such as groupers, requires the use of rotifers with a maximum body size smaller than 100µm. “Super Small” rotifer strains such as Brachionus rotundiformis Tschugunoff have been applied for this purpose.
Rotifer cultures on microalgae strains with a small cell size have been earlier used to decrease average size of rotifer populations (Knuckey, 2004). ÎThe aim of this work was to determine the influence of different strains of microalgae on average body size of the common rotifer strain Brachionus plicatilis.
However, the size of rotifers depends upon various parameters such as temperature, life stage, and type of food.
Æ Table 2: Experimental setup 5 days 25°C 30 ppm 250 ml Erlenmeyer 10 rotifers/ml None
4.38 2.80 1.65 3.52 3.02
24.11 7.20 7.21 11.08 7.07
Media exchange
100% daily with 30µg algae DW/ml
The microalgae strains used (table 1) were cultured under the following conditions: F2 medium, 30 ppt salinity, 24 hours illumination, 22°C, and harvested only during the exponential phase. Rotifer cultures started using pure strains of Brachionus plicatilis, previously fed ad libidum with Tetraselmis suecica. Experimental cultures were set as describe in table 2. Rotifers were daily filtered using a 35 µm mesh, and daily fed with a constant number of algae cells (30 µg algae DW/ml).
1.00
Micro N gad N occ
Eggs/ind
0.75
150
Rotifers’ loricas length and width were determined at the beginning (day 0) and at the end of the experiment (day 4), using a minimum of 30 egg-carrying rotifers from each culture.
0.50
0.25 Ind/mL
Dry weight (pg per cell) 161.26
Materials and methods:
Experiment duration Temperature Salinity Culture container Initial inoculation Aeration
Population density and egg ratio were daily determined.
100 0.00 0
1
2
3
4
Days
50
ŠEgg ratio and concentration à obtained along the experiment.
0 0
1
2
3
4
Æ
Days
Width and lorica length at the beginning and end of the experiment.
150
225 Lorica width Lorica length (μm)(µm)
50
0
*
*
175
*
*
150 125
53%
Î A 10% decrease in both lorica length (158µm ± 2.4 SD) and width of rotifers (117µm ± 1.6 SD) occurred when feeding M. pusilla. Î A significant change (p
tional time on a PC based on a Pentium III, 667 Mhz. As expected ... 0.2. 0.4. Example from Paper [2] time. 0. 5. 10. 15. 20. 25. 200. 300. 400. 500. # var up. 0. 5.
Environment International 26 (2001) 131±135. 0160-4120/01/$ ± see front matter D 2001 Elsevier Science Ltd. All rights reserved. PII: S0160-4120(00)00100-8 ...
Chemical gases: liquefied inflammable gases: unsaturated and .... Sulfur Dioxide - SO2. 1.1763 ... silicone vapours, halogens the sensor can lose all sensitivity.
Received 31 July 1997, accepted 5 September 1997 rod on whose visible face the adjective either 'long' or 'short' was printed. We expected an effect of the ...
1 INTRODUCTION. Conventional, or "static" fault trees are not at all suited for modeling systems in which there are strong dependencies between components.
Oct 7, 2004 - Set of test-cases in the field of system dependability assessment ... proposed in the test-cases activity of the âMethodological research working .... This case considers a simple fault-tree, where five components occur ..... A synops
considerably reduce the combinatorial explosion problem ..... reduces the combinatorial problems in the se- ... analytic method, the computation time needed to.
free of charge. To become a member ... Members are entitled to a 5% discount on the passage ... within countries of the EU an ID card is sufficient). If your final.
May 22, 2000 - and isoproturon along a contamination gradient. Ursula Dorigo ... composition of the communities sampled was assessed by microscopy and by HPLC pigment analysis. ..... impossible, even by co-eluting the sample with the.
The Falco owners' manual calls out two acceptable spark plugs. Both are NGK ... by other manufacturers (Denso, perhaps) that have the same reach and heat range, but there is ...... Remove the spring compressor, and screw the preload cap.
ence algorithm was used to calculate peak velocities. ... magnetic field-search coil technique that has linearity ... different in the first two 10-deg intervals. These.
using manual segmentations. The robustness of the ... the segmentation approaches with respect to the real user objective. ... participants in the MICCAI 2009 Grand Challenge by Sun- nybrook ... H1: The statistical distribution of the true value Îp
The subject fixated either in primary position (head and eyes are oriented in the ... 20~ calibrations were linear over the entire range of eye movements tested(up to 45~ ...... Von Hoist, E., Mittelstaedt, H. : Das Reafferenzprinzip. (Wechselwir-.
suspected cognitive disorders in adults unless the accused is con- sidered unfit to stand trial or not criminally responsible by rea- son of a mental disorder.
Integers, negative, positive. An integer is just a number with no decimal part. It's positive (sign +) if it's greater than 0 and negative (sign -) if it's lower than 0.
Embedded Computing Laboratory,. Gif-sur-Yvette ... an asymmetric MPSoC architecture with a centralized controller ... and exploit the parallelism at the thread level (TLP), where multiple ... processors is a critical part for the overall architecture
Ph.D., of the Squibb Institute for Medical Re- ... Visual Science, Yale University School of Medicine, .... repeating the procedures several times to obtain sufficient ...
of the pig tendon increased through 12 months 'endurance training'. In contrast ... following isometric training using longer duration contractions compared to shorter ..... of aerobic and anaerobic training on collagen fibers of tendons in rats.
The trend of both the experimental and simulation results (at the drift field .... the periodic hole pattern, the field lines from the drift region are compressed in the ...
of the member is the usual purpose of muscular action and move- ment is often taken as the .... One might be inclined to say that the chemical changes which.
during pointing movements involving the trunk in healthy and hemiparetic ... (DFs) of the body so that, in theory, a given task can be executed in different ways.
