DNA nanotechnology: structure, computation and networks
André
Estévez‐Torres
Laboratoire
de
photonique
et
de
nanostructures
CNRS,
Marcoussis,
France
What Nature can do with nanotechnology
10
µm
A
white
globule
chasing
a
bacterium
From
D.
Rogers
1950s
(Vanderbilt
University)
Downloaded
from
hPp://www.youtube.com/watch?v=JnlULOjUhSQ
Structure: The ribosome
25
nm
Korostelev
et
al
PNAS
2007
Structure: E. coliʼs flagellum motor
Francis
et
al
J.
Mol.
Biol.
1994
Alberts
et
al
Essen*al
Cell
Biology,
3rd
Edi^on
downloaded
from:
youtube.com/watch?v=Ey7Emmddf7Y
Structure: DNA double helix
Watson
and
Crick,
Nature
1953
Structure: DNA double helix
≈10
bases
≈
3.4
nm
Watson
and
Crick,
Nature
1953
≈2
nm
Structure: DNA double helix
≈10
nm
≈2
nm
Computation
Networks
Operon lactose
Tyson,
Annu.
Rev.
Phys.
Chem.
2010
DNA nanotech
Structures
Seemanʼs idea (1982)
La
profondeur.
M.C.
Escher
Seeman,
J.
Theo.
Biol.,
1982
How?
Hollyday,
Gen.
Res,
1964
First DNA nano-object: A cube (1991)
20
bases
≈
7
nm
Chen
and
Seeman,
Nature,
1991
2D crystals (1998) AFM
image
300
nm
Winfree,
Lieu,
Wenzler
and
Seeman,
Nature,
1998
DNA length and salt 1
µM
100
µM
10
mM
3
M
Increasing
monovalent
salt
concentra^on
Makita
et
al
Macromolecules
2006
M3+ salt induce DNA condensates Compact
DNA
Coiled
DNA
2
µm
•
Slow
transla^onal
diffusion
•
High
intra‐chain
fluctua^ons
•
Fast
transla^onal
diffusion
Videos
by
A.
Estévez‐Torres,
Kyoto
University,
2007
•
No
intra‐chain
fluctua^ons
DNA condensates Cryoelectron
microscopy
50
nm
λ
DNA
condensed
with
Co(NH3)3+
200
µM
Hud
and
Downing
,
PNAS,
2001
Reversible DNA condensation (2006)
5
µm
Le
Ny
and
Lee,
J.
Am.
Chem.
Soc.
2006
Reversible DNA condensation
for gene regulation (2010)
Estévez‐Torres
and
Baigl,
SoA
MaBer,
2011
Reversible DNA condensation
for gene regulation (2010)
Estévez‐Torres
et
al,
PNAS,
2010
DNA origami (2006)
backbone:
M13
ssDNA
(7176
nt)
staples:
32
nt
Rothemund,
Nature,
2006
DNA origami (2006)
Shawn,
Wyss
Ins^tute
Downloaded
from
hPp://wyss.harvard.edu/viewmedia/32/dnaorigamibased‐self‐assembly
100
nm
Rothemund,
Nature,
2006
3D origami
Douglas
et
al,
Nature,
2009
A nanobox with a controllable lid (2009)
Andersen
et
al,
Nature,
2009
Reconfigurable DNA canvas (2012)
150
nm
17
h,
yield:
6‐40
%
Wei,
Dai
and
Yin,
Nature,
2012
DNA nanotech
Computation
Computation with DNA (1994)
Adleman,
Science,
1994
Algorithmic self assembly (2004) Sierpinski
triangle
W.
Sierpinski,
1915
Rothemund
et
al,
Plos
Biol,
2004
DNA nanotech
Networks
DNA-fuelled DNA machine (2000)
Yurke
et
al,
Nature,
2000
Walkers (2004)
Shin
and
Pierce,
J.
Am.
Chem.
Soc.
2004
Chemical synthesis (2010)
He
and
Liu,
Nature
Nanotech,
2010
DNA strand-displacement logic gates (2006)
Seelig,
Soloveichik,
Zhang
and
Winfree,
Science,
2006
DNA strand displacement and
chemical kinetics (2010) Species
A
Barcode
Species
B
Soloveichik,
et
al,
PNAS,
2010
DNA strand displacement and
chemical kinetics
Validated
in
simula^ons
only
Soloveichik,
et
al,
PNAS,
2010
Introducing time-responsiveness
with enzymes (2011) α
"
β
:
α
ac^vates
β
Montagne
et
al,
Mol.
Sys.
Biol.,
2011
The first engineered chemical oscillator (2011)
Montagne
et
al,
Mol.
Sys.
Biol.,
2011
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