Phosphorylated Sugars by LCMS UDP, ADP, TDP and CDP Method Conditions Column: Catalog No.: Dimensions: Solvents:
Notes: Please note the addition of small amount of ammonia to the sample matrix. The alkaline environment of the sample matrix assured efficient and symmetrical peaks for all analytes.
Cogent Diamond Hydride™ 4µm, 100Å. 70000-15P-2 2.1 x150 mm A: DI water + 0.1% ammonium formate pH 7.2 B: 90%acetonitrile + 10% DI water + 0.1% ammonium formate pH 6 Mobile phase: Gradient: Time %B Time %B 0.0 95 12.1 95 10.0 75 15.0 95 12.0 75 Flow rate: 0.3 µL/min. Peaks: 1. ADP – glucose, RT = 3.68 min, monitored MRM transitions were m/z 588 to m/z 346
2. Proprietary sugar nucleotide, 3.
Discussion
4.
UDP hexanolamine was used as the internal standard in the analysis of nucleotide sugars in this rapid analysis method. The mobile phase was designed to maximize the detector response in LC-MS for maximum efficiency. The simple “inverse gradient” which produces ANP (aqueous normal phase) HPLC method was required for the results shown therefore a Cogent Diamond Hydride column was chosen. This method can be used in measuring metabolite concentration.
5.
RT = 6.03 min, monitored MRM transitions were m/z 563 to m/z 321 Proprietary sugar nucleotide, RT = 6.20 min, monitored MRM transitions were m/z 606 to m/z 385 CDP – glucose, RT = 7.13 min, monitored MRM transitions were m/z 564 to m/z 322 UDP hexanolamine (internal standard), RT = 8.40 min, monitored MRM transitions were m/z 502 to m/z 258
MRM – multiple reaction monitoring in LC/MS/MS Sample Preparation: 400 mL DI water+400 mL acetonitrile+20 mL of stock solution of each compound + 5 mL of 12% ammonia. Detection: ESI – neg - Agilent 6210 MSD TOF mass spectrometer.
The upper chromatogram is the initial separation, ... conditions that would hydrolyze other reversed phases. See next page for ... silica in reversed phase.
Description. APP A44. APPLICA. TION. NOTES. Method Conditions. Column: ... retention higher percentages of water in the mobile phase can be used. The diminished hydrophobicity is shown in Table 1. For more information visit www.
as base) bound to a ribose or deoxyribose sugar. Examples of these include cytidine, uridine, adenosine, guanosine, thymidine and inosine. Nucleosides can ...
Discussion. The polar compound, Metformin, and the nonpolar compound. Glyburide, can be retained on the same stationary phase (see A. & B), utilizing the ...
40018-75P. Cogent Bidentate⢠C18 HPLC Column, 4µm \,100A 4.6mm x 75mm. Cat. ... Solvent A: 100% DI water + ... 1mg of the compound dissolved in 1 mL.
for melamine: - Agilent 6210 MSD TOF mass spectrometer. Note: When an instrument with ... Use of LC-MS detection eliminates the need for derivatization (as is ...
Mass Spec: Atmospheric Pressure. Chemical Ionization in positive mode: APCI+ Single Ion Monitoring. Discussion. Two proprietary compounds, which are ...
Cholesterol⢠HPLC columns, peaks ETES and ESTN are resolved with polarity-based, Reverse Phase, ACN as the organic modifier. ⢠With other C18 columns, ...
This active pharmaceutical ingredient was separated from related compounds in a real life pharmaceutical preparation. Note the excellent peak shape and the ...
Cogent Diamond Hydride⢠HPLC Column, 100à , 4µm, 2.1 x 150mm. Cat. No. ... The analysis of succinic acid is mostly done using gas chromatography. (GC) ...
functions, reduction of cholesterol level and reduction of the risk of cancer and the mprovement of digestion/absorption of food ingredients such as minerals.
Cogent Silica-C⢠HPLC Column, 100à , 4µm, 4.6 x 75 mm. Cat. No. Description ... determination of amino acids in many types of samples. Ordinary columns ...
Cogent Bidentate C18 HPLC Column, 100à , 4µm, 4.6 x 75 mm, Standard End Fittings. Cat. No. ... refraction indices between solvent A and solvent B in gradient ...
69069-Q20. Cogent Qx2⢠Column for LC-MS, 2.1 ID x 20 mm, PEEK & Titanium housing, ... Mass Spec: Atmospheric Pressure. Chemical Ionization in positive.
determination can be used for diagnosis of galactosemia in newborn babies [1-3]. Literature: [1]. Ji-Seon Jeong, Ha-Jeong Kwon, Hye-Ran Yoon, Yong-Moon ...
In contrast when a market leading HILIC column was used under the same conditions, the efficiency dropped by more than 30% over this same range of flow ...
Mass Spec: Atmospheric Pressure. Chemical Ionization in the. Positive mode: APCI+. Discussion: Major Peaks. Table below: Major peaks in mass spectra of ...
