ESI â neg - Agilent 6210 MSD TOF mass spectrometer. Discussion ... depending on individual instrument configuration. It is important to remember not to ...
Cogent Diamond Hydride™ 4µm, 100Å. 70000-15P-2 2.1 x150 mm A: 50% Methanol / 50% Water / 0.05% Formic Acid (strong solvent) B: 90% Acetonitrile / 10% Water w/10mM Amm Acetate (weak solvent) Linear gradient over 10 minutes. Adjustable per instrument.
Post Tme: Flow Rate: Injection: Samples:
5 min. 0.4 mL/min. 1 microL 1. Maleic Acid, 115 m/z (M - H)2. Fumaric Acid, 115 m/z (M - H)Sample stock solutions were made in acidified DI water at a concentration of 0.2 mg/mL Samples for analysis were made by diluting the stock 1:100 in 50:50 solution A and B
Detection:
ESI – neg - Agilent 6210 MSD TOF mass spectrometer
Discussion Two isobaric acids, maleic and fumaric (115 m/z) are separated very well when using the solvents above and a gradient method. Solvent A used in this application note contains 50% methanol which is recommended when biological samples are used. This method is very reproducible and is very fast to equilibrate between gradient runs with 0.08%RSD. Maleic and fumaric are simple acids and the peak shape of these acids is not affected by the presence of sodium in the system as with more complex acids. When analyzing complex compounds, it is recommended that as much sodium be removed from the system as possible (i.e. replacing glass bottles with Teflon bottles) or the mobile phase should be prepared fresh daily. Note: The gradient used for this application note was a simple linear gradient over 10 minutes. The exact conditions are not provided since they will have to be adjusted depending on individual instrument configuration. It is important to remember not to overload the column or saturate the detector (it is recommended to adjust the sample concentration so that injection is between 1 & 5 microL).
Mass Spec: Atmospheric Pressure. Chemical Ionization in the. Positive mode: APCI+. Discussion: Major Peaks. Table below: Major peaks in mass spectra of ...
Chromatogram C shows the retention and elution of. Carvone in organic ... Bidentate C18⢠column, a quick âstep gradientâ or change of mobile ... âPrep upâ in NP and âCheck Purityâ in RP on the Same Analytical Column. HPLC. 03-H-08-A1.
Reverse Phase HPLC is employed for the initial analysis and the final large scale purification of peptides. The first step of the production of ... chromatogram.
The upper chromatogram is the initial separation, ... conditions that would hydrolyze other reversed phases. See next page for ... silica in reversed phase.
Description. APP A44. APPLICA. TION. NOTES. Method Conditions. Column: ... retention higher percentages of water in the mobile phase can be used. The diminished hydrophobicity is shown in Table 1. For more information visit www.
as base) bound to a ribose or deoxyribose sugar. Examples of these include cytidine, uridine, adenosine, guanosine, thymidine and inosine. Nucleosides can ...
Discussion. The polar compound, Metformin, and the nonpolar compound. Glyburide, can be retained on the same stationary phase (see A. & B), utilizing the ...
40018-75P. Cogent Bidentate⢠C18 HPLC Column, 4µm \,100A 4.6mm x 75mm. Cat. ... Solvent A: 100% DI water + ... 1mg of the compound dissolved in 1 mL.
for melamine: - Agilent 6210 MSD TOF mass spectrometer. Note: When an instrument with ... Use of LC-MS detection eliminates the need for derivatization (as is ...
Mass Spec: Atmospheric Pressure. Chemical Ionization in positive mode: APCI+ Single Ion Monitoring. Discussion. Two proprietary compounds, which are ...
Cholesterol⢠HPLC columns, peaks ETES and ESTN are resolved with polarity-based, Reverse Phase, ACN as the organic modifier. ⢠With other C18 columns, ...
This active pharmaceutical ingredient was separated from related compounds in a real life pharmaceutical preparation. Note the excellent peak shape and the ...
Cogent Diamond Hydride⢠HPLC Column, 100à , 4µm, 2.1 x 150mm. Cat. No. ... The analysis of succinic acid is mostly done using gas chromatography. (GC) ...
functions, reduction of cholesterol level and reduction of the risk of cancer and the mprovement of digestion/absorption of food ingredients such as minerals.
Cogent Silica-C⢠HPLC Column, 100à , 4µm, 4.6 x 75 mm. Cat. No. Description ... determination of amino acids in many types of samples. Ordinary columns ...
Cogent Bidentate C18 HPLC Column, 100à , 4µm, 4.6 x 75 mm, Standard End Fittings. Cat. No. ... refraction indices between solvent A and solvent B in gradient ...
