Physiological Data Summary – C57BL/6J (000664) Data also available from the Mouse Phenome Database (MPD, www.jax.org/phenome) Body Weights Age (weeks) Female Mean SD Male Mean SD

3 4 5 6 7 8 9 - 12.26 15.51 16.65 17.54 18.28 18.91 - 1.61 1.49 1.31 1.32 1.38 1.43 - 13.59 17.93 20.39 22.42 23.73 24.84 - 1.59 1.43 1.43 1.40 1.47 1.61

Age (weeks) 10 11 12 13 14 15 16 Female Mean 19.63 20.09 20.58 21.21 21.67 21.99 22.15 SD 1.55 1.60 1.69 1.80 2.17 2.12 2.09 Male Mean 25.97 27.01 27.82 28.49 29.18 29.71 29.97 SD 1.70 1.57 1.58 1.69 1.75 1.85 1.87 Approximately 100 mice of each sex were obtained at weaning (BD +/1 day) from production rooms individually identified and weighed the same day every week. The mice were fed a diet containing 6% fat (LabDiet® 5K52/5K67) from weaning at 4 weeks until 16 weeks of age.

Parameter Hematology Age White blood cell count (WBC) Red blood cell count (RBC) Hemoglobin Hematocrit Mean cell volume (MCV) Mean cell hemoglobin (MCH) Mean cell hemoglobin concentration (MCHC) Platelet count Mean platelet volume (MPV) Percent reiticulocytes Reticulocyte hemoglobin Reticulocyte count Percent neutrophils Percent Lymphocytes Percent Monocytes Percent Eosinophils Percent Basophils Neutrophil count Lymphocyte count Monocyte count Eosinophil count Basophil count Biochemistry Albumin Total protein Blood urea nitrogen Calcium Phosphorous Cholesterol HDL cholesterol Triglycerides Free fatty acids Glucose Alanine tranferase

Units

Females

Weeks

8

16

8

16

3

3.48

2.67

2.62

3.10

6

10.77 17.0 51.5

10.90 16.4 51.2

10.59 16.20 52.1

10.62 16.0 51.8

fL

47.8

47.0

49.2

48.8

pg

15.8

15.1

15.4

15.2

g/dL 3 10 cells/µL

33.2 1019

32.1 1085

31.2 1157

31.1 1310

fL

6.7

6.6

7.0

6.4

%

3.6

3.1

3.8

2.9

15.8 387.9 7.4

15.5 335.7 8.3

15.8 400.8 18.5

15.1 302.7 33.9

% % % % 3 10 cells/µL 3 10 cells/µL 3 10 cells/µL 3 10 cells/µL 3 10 cells/µL

88.6 0.7 2.6 0.2 0.25 3.08 0.03 0.09 0.01

86.6 1.0 3.2 0.2 0.23 2.32 0.03 0.08 0.00

76.7 1.4 2.6 0.2 0.52 1.98 0.04 0.06 0.01

61.6 2.0 1.7 0.2 1.17 1.80 0.06 0.05 0.01

g/dL g/dL mg/dL mg/dL mg/dL mg/dL mg/dL mg/dL mEq/L mg/dL IU/L

3.9 6.1 27 10.6 10.3 79 67.2 93 1.72 176 43

4.2 6.4 22 10.4 10 70 58.5 67 1.69 152 40

3.7 6.0 24 10.4 10.3 100 89.9 80 2.07 156 57

3.9 6.4 27 10.9 9.3 93 79.7 71 1.81 159 79

10 cells/µL 10 cells/µL g/dL %

pg 9 10 cells/L %

Males

Creatine kinase Thyroxine/T4

IU/L µg/dL

Parameter Units Organ Weights Age Weeks Brain g % of body weight Heart g % of body weight Liver g % of body weight Left kidney g % of body weight Right kidney g % of body weight Spleen g % of body weight

720 5.7

358 6.1

Females

799 4.9

601 4.5

Males

8 0.417 2.35 0.109 0.61 0.913 5.12 0.103 0.58 0.159 0.90 0.072 0.41

16 0.415 1.89 0.145 0.66 1.038 4.72 0.117 0.53 0.134 0.61 0.081 0.37

8 0.423 1.75 0.151 0.62 1.271 5.25 0.142 0.58 0.149 0.61 0.084 0.35

16 0.420 1.38 0.200 0.66 1.444 4.74 0.164 0.54 0.174 0.57 0.080 0.26

Body Composition by DEXA Analysis g; Total DEXA body weight Tissue 17.70 2 Bone mineral density g/cm 0.044 Bone mineral content g 0.327 2 Bone area cm 7.73 Lean tissue g 14.6 Fat tissue g 3.1 Percent fat tissue % 17.7

19.91 0.050 0.404 8.04 15.9 4.0 19.8

21.88 0.046 0.373 8.12 18.1 3.8 17.4

27.08 0.055 0.462 8.45 22.5 4.6 16.8

56.13

67.12

57.80

-

15.51

12.22

13.60

-

9.99

8.37

8.63

-

1.93

1.10

1.94

-

0.98

0.89

1.12

-

0.93

0.78

1.26

-

3.71

2.94

4.24

-

Flow Cytometry - Spleen Lymphoid cells B cells B cells (B220+) % T cells CD4 T cells (CD3+, CD4+) % CD8 T cells (CD3+, CD8+) % NK cells (CD3-, NKG2D+) % NKT cells (CD3+, NKG2D+) % Myeloid cells Granulocytes (MAC1+, GR1+) % Monocytes (MAC1+, GR1-) %

Physiological Data Summary – C57BL/6J (000664) Data also available from the Mouse Phenome Database (MPD, www.jax.org/phenome) For 8 week data, mice of each sex were obtained at 8 weeks of age (BD +/- 3 days) from production rooms. For 16 week data, mice of each sex were obtained at weaning (BD +/- 1 day) from production rooms and maintained until 16 weeks of age. All measurements are non-fasted values.