Gigsaw Next Gen Sequence Assembly Simulator ... Make sure that reads sheet 1 forward is on the back of the same sheet of paper as reads sheet 1 reverse and.
Gigsaw Next Gen Sequence Assembly Simulator Print out the sequence reads and reference sequence sheets. Make sure that reads sheet 1 forward is on the back of the same sheet of paper as reads sheet 1 reverse and ensure that the reads align. Cut out the reads along the white horizontal gaps. &ŽƌƉĂŝƌĞĚĞŶĚƌĞĂĚƐ͕ĐĂƌĞĨƵůůLJĚŝǀŝĚĞƚŚĞŵĂƐƐŚŽǁŶŝŶƚŚĞĮŐƵƌĞďĞůŽǁ
The reference sequence can be assembled by using the grey box on the row end as a tab on which ƚŽƐƟĐŬƚŚĞƉƌĞǀŝŽƵƐƐĞƋƵĞŶĐĞƉŽƌƟŽŶ;ƉŽƐŝƟŽŶƐĂƌĞŶƵŵďĞƌĞĚĂďŽǀĞƚŚĞƐĞƋƵĞŶĐĞͿ͘
For a normal assembly, try to overlap the reads as much as possible. You should end up with approximately ƚŚĞƌĞĂĚĚĞƉƚŚŽĨƌĞĂĚƐĂƚĂůůƉŽŝŶƚƐŝŶƚŚĞĮŶĂůĂƐƐĞŵďůLJ͘ZĞŵĞŵďĞƌƚŚĂƚƚŚĞƌĞǀĞƌƐĞĐŽŵƉůĞŵĞŶƚƐĞƋƵĞŶĐĞ ;ƚŚĞŽƚŚĞƌƐƚƌĂŶĚŽĨEͿĐĂŶďĞĨŽƵŶĚŽŶƚŚĞƌĞǀĞƌƐĞƐŝĚĞ͘
Read depth 1 2 3 4 3 2 1 &ŽƌƉĂŝƌĞĚĞŶĚƌĞĂĚƐ͕ƉůĂĐĞƚŚĞŵĨĂĐŝŶŐĞĂĐŚŽƚŚĞƌĂƉƉƌŽdžŝŵĂƚĞůLJƚŚĞŐĂƉƐŝnjĞĂƉĂƌƚ͘ŐĂŝŶĂƩĞŵƉƚƚŽĂůŝŐŶ them as well as you can.
gap width
&ŽƌĂůŝŐŶŝŶŐĂŐĂŝŶƐƚƚŚĞƌĞĨĞƌĞŶĐĞƐĞƋƵĞŶĐĞ͕ƚƌLJƚŽĮŶĚƚŚĞƉŽƐŝƟŽŶǁŚĞƌĞƚŚĞƐĞƋƵĞŶĐĞďĞƐƚŵĂƚĐŚĞƐ͘/ĨLJŽƵĂƌĞ looking for SNPs or have a high error rate then there may not be a perfect match. SNP
Variant
possible sequence error
reference Gigsaw created by Dr David Martin, University of Dundee, Scotland
Gigsaw Parameters for Lea PLUS Sequence Length
92
Coverage
4.6
Error rate
30/1000 bases
Polymorphisms
Sequence contains 0 polymorphisms
Gigsaw created by Dr David Martin, University of Dundee, Scotland
Lea PLUS sheet 1 forward reads
C C C A G G G C C T C A C C A A C A C C T C C A C A G G G C A G T A A A G G C A G A A T C C A C G T T C A C C T T G C C C C A C A G A C T T C T A C T C A G G A G T C A C T C C T C A G G A G T C A G A T G C A C C C A C A G C G C A G T A A C G G C A G A C A G G G C C T C A C C A C C A A C T T C T G C C C C A C A G G G C A G T A A C G G A C T C C T G A G G A G A A G T C T G C C C G T T C A C C T T G C C C C A C A G G G T G C A T C T G A C T C C T G A G G A G A
X A T G G A G C A T C T G A C T C C T G A G A T C C A C G T T C A C C T T G C C C C A T G T G G G G C A A G G T G A A C G T G G A C G T G G A T G A A G T T G G T G G T G C C C A G G G C T T C A C C A C C A A C T C A G G G C C T C A C C A C C G A C T T C T G A A G T T G G T G G T G A G G C C C T A A G T C T G C C G T T A C T G C C C T G C A T C T G A C T C C T G A G G A G A A G Gigsaw created by Dr David Martin, University of Dundee, Scotland
Lea PLUS sheet 1 reverse reads
A G G T G T T G G T G A G G C C C T G G G T C T G C C T T T A C T G C C C T G T G G T G G G G C A A G G T G A A C G T G G A T T G A C T C C T G A G T A G A A G T C T G G T G C A T C T G A C T C C T G A G G A G T C T G C C G T T A C T G C G C T G T G G G A A G T T G G T G G T G A G G C C C T G C C G T T A C T G C C C T G T G G G G C A G G C A G A C T T C T C C T C A G G A G T C C C T G T G G G G C A A G G T G A A C G T C T C C T C A G G A G T C A G A T G C A C T C A G G A G T C A G A T G C T C C A T T G G G G C A A G G T G A A C G T G G A T C C A C G T T C A C C T T G C C C C A C A C A C C A C C A A C T T C A T C C A C G T A G T T G G T G G T G A A G C C C T G G G G A A G T C G G T G G T G A G G C C C T G A G G G C C T C A C C A C C A A C T T C A C A G G G C A G T A A C G G C A G A C T T C T T C T C C T C A G G A G T C A G A T G Gigsaw created by Dr David Martin, University of Dundee, Scotland
Read depth 1 2 3. 4. 3 2 1 gap width reference. SNP. Variant possible sequence error. Gigsaw created by Dr David Martin, University of Dundee, Scotland ...
Jun 3, 2010 - tion of related molecules has been linked with the emergence of .... a, Representation of the linear and circular forms of the EsV-1 genome.
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live insects, bird excrement, and sweet food waste (10). That adult ants are able to live ... with honey water and cockroaches. ... DNase I before further treatment.
Activation of DNA cleavage may be regulated further by the topologically ... Active DNA synthesis during the second cell cycle accounts for the final .... through the first cell cycle, after several rounds of genome amplification .... catalytic subun
mites))3,4 (Supplementary Fig. ... 1Department of Biology, The University of Western Ontario, London N6A 5B7, .... RNA-seq data on matching samples (Supplementary Note 2.3). ..... The T. urticae and Acyrthosiphon pisum sequences form a.
genome, representing the first complete chelicerate genome. ... environment and organization of the Hox complex, and also reveals evolutionary innovation of silk production. ... Lower Devonian period (410 million years ago). ... Some mites are of maj
He has also written song lyrics for various ... York City with his wife, two sons, and a golden retriever named Alice. ... LOUISA HALL studied English at Harvard and has a PhD in. Literature ..... during World War IIâa true story that has already s
Thailand (Freeform), UK (Transworld), US (Bloomsbury), Film (UFA Film & TV. Production). .... and internal conflicts; their strategies for survival and responses to setbacks. ..... deep horror is that this man is normal, that in other circumstances h
I think we will have time for a coffee »,. â « There'll probably be lots of people at ... I've got some shopping to do ». 4/Will or be going to? â « It's going to rain.
The Prince of Wales » / « The Queen of Egypt » / « The King of Jordan » / « The President of. France » BUT « Queen Elizabeth II » / « King Hussein ...
life has not been the same for Andrew Stilman. . . On the morning .... He crafts smooth sentences and can make you laugh out loud without ever undermining or selling ...... published various academic studies on Buddhism as well as The Gate ...
We are decorating the flat » BUT NOT « I (am owning) a flat » â « I own a flat ». â Not «I (am ... The ferry gets into Rotterdam at six o'clock tomorrow morning », ... I'm reading an interesting book at the moment, but I can't remember wha
Prosecutor David Andrews said Wilson had stolen a gold wedding ring and credit card and had used the card to attempt to withdraw money from a bank. He had ...
Poland â Polish â a Pole. Spain â Spanish â a Spaniard. 3D. England â English â a Englishman/Englishwoman â English people / The English.
7/Use of the past perfect continuous ... 3/ William travaille sur son rapport de stage depuis 2 heures de l'après-midi. Il a déjà ... 11/Il avait déjà fait 3 ans de prison.
