Basis of Immunology and Immunophysiopathology of Infectious Diseases
TD-BF02 AS
TD-BF02: Activation of T and B lymphocytes I. The carrier effect (from Mitchison et al. 1969, Raff et al. 1970)
Mice were immunized (stimulus 1) with NIP-OVA, SAH, SAB or not immunized (-). Spleen cells were used untreated (Expérience 1) or treated with antibodies in the presence of complement (Expérience 2) and transferred into irradiated syngeneic host mice. Recipient mice were then immunized again (stimulus 2) with NIP-OVA, NIP-SAB, (NIP-SAB + SAH) or (NIP-SAH + SAB). Anti-NIP response is measured seven days after stimulation by the technique of plaque forming cell response1. Results are presented in the table below: Stimulus 1: stimulus of transferred cells
Treatment received by spleen cells
Donor I
Donor II
Donor I
Donor II
1. NIP-OVA
-
-
-
Stimulus 2
AntiNIP response after stimulus 2
NIP-OVA
+
2. NIP-OVA
-
-
-
NIP-SAB
-
3. NIP-OVA
SAB
-
-
NIP-SAB
+
4. NIP-OVA
-
-
-
NIP-SAB+SAH
-
5. NIP-OVA
SAB
-
-
NIP-SAB+SAH
+
6. NIP-OVA
SAB
-
-
NIP-SAH+SAB
-
7. NIP-OVA
SAH
-
-
NIP-SAB+SAH
-
8. NIP-OVA
SAH
-
-
NIP-SAH+SAB
+
9. NIP-OVA
SAB
SSNI+C’
SSNI+C’
NIP-SAB
+
10. NIP-OVA
SAB
SSNI+C’
Anti-Thy1+C’
NIP-SAB
-
11. NIP-OVA SAB Anti-Thy1+C’ SSNI+C’ NIP-SAB + Legend: Hapten Æ NIP (4-hydroxy 5-iodo 3-nitrophenacetyl) Carrier Æ OVA (ovalbumin), SAH (human serum-albumin), SAB (bovine serum-albumin) SSNI Æ non-immunized mouse serum
Question 1.
Analyze the results presented in these experiments in the light of cooperation in the immune system.
1 The technique of plaque forming cell reveals the number of antibody-producing cells. Spleen cells isolated from recipient animals are plated on Petri dishes containing NIP-conjugated red blood cells and complement. Anti-NIP antibodies diffuse around antibody-producing cells causing the lysis of NIP-conjugated red blood cells, therefore forming plaque around the producing cells.
Institut Pasteur in Ho Chi Minh City, Vietnam, January 24 – February 5, 2005
1
Basis of Immunology and Immunophysiopathology of Infectious Diseases
Institut Pasteur in Ho Chi Minh City, Vietnam, January 24 – February 5, 2005
1. In this study we have recorded the activ- ity of motor units of the important ... flexor muscles under varying conditions ( 1,7, ...... Basel, Karger, 198 1, vol. 9, p.
T. S., ALMDALE,. D. P.J., LEWIS,. J. L.,. AND RYMER,. W. Z. Characteristics of synergistic re- lations during isometric contractions of human el- bow muscles.
Unique signal transduction through TCR and FceRIγ in type b IELs. Type b IELs ..... RORγt, an orphan nuclear hormone receptor, was detected in immature DP ...
Provocatively, the disjunction between the high expression of ..... MacDonald,T. & Ferguson,A. Small intestinal architecture and protozoal infection in mice.
via amygdala and cortico-basal ganglia loops [9]), the program projected to the ... consequences of the autonomic evaluation effect, Pers. Social Psychol. ... Attention and Orienting: Sensory and Motivational Processes, Lawrence. Erlbaum ...
release assays at four effector to target cell ratios (E:T). Shown are representative ... c Enrichment by anti-V1-coated magnetic beads. d MAGE-A1 peptide ..... phocytes produce cytokines, specifically lyse tumor cells, and recycle lytic capacity.
contracting muscle fibers grows, there would be progressive engagement of more tendon strands so that tendon stiffness would increase ... section beryllium.
across different experiments in single subjects, was identified on only one side in ... The data were analyzed by pixel-to-pixel Student's paired t-test such that the ...
Dec 13, 2017 - devices,set top boxes and other embedded devices, as well as . ... milUon), and willhite such mmmgemc;nt,marke1ing and~{ftwlP"C ... development community, whether by means of purchase by cash or shares, ~r merger, ... such funding, and
Apr 9, 2004 - and MyHC expression in pure and hybrid fibre types. J Muscle Res Cell ... Westing SH, Cresswell AG, and Thorstensson A. Muscle activation.
If the answer is positive, it could be applied as a design criterion for VI NES to control the ... A LO under periodic excitation and coupled with a VI NES is showed in Fig. ...... Table 2. Simulation parameters for a cutting tool coupled with VI NES
both free Ca 2+ concentration in the SR and depolariza- tion of T-tubule (V'). A charge deposited on the. T-tubular membrane by a received action potential.
Feb 24, 1994 - extensions as rapidly as possible from an initial position to a visually defined target at 36â in amplitude. In electromyograms, the reciprocal ...
in time, and âinvertedâ (or negated to produce a reciprocal pattern). These results .... amounts to calculating PC 1' and PC2' in the following form. PCl' = aPC1 + .... higher levels of coactivation (of all muscles) and prolonged activation after
Feb 13, 2004 - carbohydrate diet (30) and in Pima Indians predisposed to Type. 2 diabetes (26). .... PPAR-α protein quantification. Samples were thawed in ...
Aug 12, 2008 - molecular dynamics simulations using an embedded atom method EAM potential are used ... a dramatic effect on the behavior of devices.1 Therefore, a ..... Electron. 3, 295 1978. 34 M. J. Mills and P. Stadelmann, Philos. Mag.
illicit opioid drugs (opium, heroin) as well as approved therapeutics (morphine and codeine) are mediated by the activation of the mu-opioid receptor (µOR).
magnetic field gradients were obtained simultaneously at 61 recording sites. ... precentral motor cortex, then was quantified by first filtering the signals through ...
Jul 4, 2007 - Other energetic blood-borne substrates, such as fatty acids and ketone ... Astrocytes are the only cell type able to perform ... acids as precursors for the synthesis of ketone bodies that are ... used our two previously described model
