Run a gel at 25V for 15 minutes and then at 100V (It is also possible to run the ... Denaturation unzips the DNA to give single-stranded molecules that have unpaired bases suitable for ... A correct transfer is achieved when all the loading blues have transferred on the membrane. ... Wash everything after use, salts crystallize.
Digest approximately 15µg of genomic DNA sample with appropriate restriction enzyme and appropriate molecular weight. Run a gel at 25V for 15 minutes and then at 100V (It is also possible to run the gel o/n at 20-25V), stain with Ethidium bromide and take a picture with a ruler (put the 0 at the slots)
