RNeasy Cleanup Protocol

3) Transfer the sample (700 μl) to an RNeasy Mini spin column placed in a 2 ml collection tube (supplied). Close the lid gently, and centrifuge for 15 seconds at ...
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Nucleic acids purification

RNeasy Cleanup Protocol

β-Mercapto-ethanol (β-ME) must be added to Buffer RTL before use. Add 10 µL β-ME per 1 mL of Buffer RTL Buffer RPE is supplied as concentrate. Add 4 volumes of 96-100% ethanol before the first use. Volume of Buffer RTL: 350-600 µL 30 µg, repeat step 7 using another 30–50 µl RNasefree water, or using the eluate from step 7 (if high RNA concentration is required). Reuse the collection tube from step 7.

Nucleic Acid Purification 20