Thursday, October 4 7:30 AM Registration 8:15 AM Welcome addresses

PLENARY SESSION : MUSCLE OXYGENATION Chairmen : M. Ferrari, T. Jue

8:30 AM

Insight into muscle oxidative metabolism gained by near infrared: problems and prospects Marco Ferrari and Valentina Quaresima Department of Biomedical Sciences and Technologies, L’Aquila, Italy; e-mail: [email protected]

University

of

L’Aquila,

67100

8:53 AM Control of Respiration at the Initiation of Muscle Contraction Youngran Chung, Paul A. Mole*, Tuan Khanh Tran, Napapon Sailasuta**, and Ralph Hurd**, Biological Chemistry and *Exercise Science, University of California, Davis, Davis, CA 95616-8635 and **GE Medical Systems, Inc., Fremont, CA 9:16 AM OXIDATIVE METABOLISM OF SKELETAL MUSCLE DETERMINED BY NEAR INFRARED SPECTROSCOPY Takafumi Hamaoka, Takayuki Sako, Motohide Murakami, Ryotaro Kime and Toshihito Katsumura. Department of Preventive Medicine and Public Health, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JAPAN 9:39 AM Skeletal muscle intracellular PO2 and exercise Russell S. Richardson1, Elizabeth A. Noyszewski2 Department of Medicine, University of California1, Department of Radiology, University of Pennsylvania

SHORT ORAL COMMUNICATIONS : MUSCLE OXYGENATION 9:45 to 11 : 00 AM 10:02 AM Muscle performance with varied extracellular PO2: from whole body to single cell. Michael C. Hogan, Creed M. Stary, Luke J. Haseler, and Russell S. Richardson Dept. of Medicine 0623A, University of California – San Diego, 9500 Gilman Dr., La Jolla, C 10:05 AM Can the P/O2 ratio be estimated in vivo in humans? Francescato Maria Pia, Cettolo Valentina, di Prampero Pietro Enrico Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Udine, 33100 10:08 AM Kinetics of oxygen uptake during exercise is dependant on carbohydrate intake N. Maassen, B. Schneider, O. Schröder, J. Mathews Department of the Sports Physiology, Medical School, Hannover, Germany. THE NORMAL RANGE OF SKELETAL MUSCLE OXIDATIVE CAPACITY EVALUATED BY NEARINFRARED 10:11 AM SPECTROSCOPY Shinya Nishio, Takafumi Hamaoka, Toshihito Katsumura Department of Preventive Medicine and Public Health, Tokyo Medical University, Tokyo, Japan 10:14 AM The Effect of Arm Position on Muscle Oxygenation Shiro ICHIMURA, Takafumi HAMAOKA Norio MURASE, Motohide MURAKAMI, Ryotaro KIME, Toshiyuki HOMMA, Chihoko UEDA, Takeshi NAGASAWA, Mitsuhiro SAMEJIMA, Yuko KUROSAWA, and Toshihito Department of Preventive Medicine and Public Health, Tokyo Medical University, 6-1-1 Shinju 10:17 AM Changes in NIR signals during constant work rate exercise M. Samejima, T. Hamaoka, S. Nishio, T. Sako, T. Homma˝S. Ichimura, T. Shimomitsu, and T. K Dept. of Preventive Medicine and Public Health, Tokyo Medical University, Tokyo, Japan 10:20 AM Methodological Aspects of In Vivo Near-Infrared Spectroscopy in Human Skeletal Muscle Mireille C.P. van Beekvelt, MSc,*† Baziel G.M. van Engelen, MD, PhD,* Ron A. Wevers, PhD,* From the *Neuromuscular Centre Nijmegen, Institute of Neurology, University Medical Centre 10:23 AM Muscle Blood Flow in Active and Inactive Young and Old Subjects. Jennifer Olive, M.S., Kevin McCully, Ph.D. Department of Exercise Science, University of Georgia, Athens, GA

30602, USA

10:26 AM A new near infrared laser-Doppler flowmeter for deep tissue perfusion monitoring T. Binzoni*, T.S. Leung†, D. Boggett§ and D. Delpy† Departments of Physiology and Radiology, Faculty of Medicine, University of Geneva, Switzerland; †Department of Medical Physics and Bioengineering, University College London, UK; §Moor Instruments Ltd, Millwey Axminster, Devon, UK. 10:29 AM Oxygen delivery in older subjects measured with near infrared spectroscopy, does race or Kevin McCully Address: Department of Exercise Science, University of Georgia, Athens GA USA Near-infrared frequency-domain mapping of vastus lateralis blood flow and oxygen 10:32 AM consumption before and after isometric contraction Valentina Quaresima1, Maria Angela Franceschini2,3, Matthew L. Hoimes2,Marco Ferrari1, and 1Dept. Biomedical Sciences and Technologies, Univ.L'Aquila, 67100 L'Aquila, Italy. Fax: 39 0862-433433 E-mail: [email protected]; 2Bioengineering Center, Dept. Electrical Engineering and Computer Science, Tufts Univ., Medford, MA 02155, USA; 3NMR-Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

COFFEE BREAK 11 : 00 to 11 : 30 AM

Thursday, October 4 (Session 2)

PLENARY SESSION : MR of function and structure Chairmen : R. Meyer, P. Styles

11:30 AM Muscle Functional MRI: Mechanisms and Applications Ronald A. Meyer Depts . of Physiology and Radiology, Michigan State Univ., East Lansing, MI, USA 11:53 AM Time resolved BOLD response in the muscle of patients with peripheral vascular occlusive dise D. Bilecen1,2, J. Winterer1, M. Büchert1, J. Hennig1 1 Section of Medical Physics, Dep. of Radiology, University Hospital Freiburg, Germany - 2 Dep. of Radiology, University Hospital Freiburg, Germany 12:16 PM Multicomponent T2 of human muscle Greg Marsh, The Lawson Health Research Institute, London, Ontario 0

SHORT ORAL COMMUNICATIONS : MR of function and structure 12:30 to 13:30 12:39 PM Functional Skeletal Muscle Imaging Using Partial k-Space MRI Adam S. Greenberg and Edward Walsh Department of Biomedical Engineering, University of Alabama at Birmingham, USA 12:42 PM Imaging of Regional Muscle Strain and Displacement Using Force-Referenced RF Tagged MR Imagin Edward G. Walsh1, Thomas Denney2, Vicki Y. Johnson3, Bradley Newcomer3 1University of Alabama/Birmingham, Department of Biomedical Engineering, 2Auburn University Department of Electrical Engineering, 3University of Alabama/Birmingham School of Nurisng, 4University of Alabama/Birmingham School of Health Related Professions 12:45 PM The effect of anticholinergic agent on the uterine myometrial contraction: evaluation on cine Asako Nakai1, Kaori Togashi2, Aki Kido1, Toshitaka Fujiwara2, Toshihide Yamaoka1, Junji Koni 1Department of Nuclear Medicine and Diagnostic Imaging, 2Hitachi Medical Corporation Chair of Department of Diagnostic and Interventional Imageology, Graduate School of Medicine, 12:48 PM Correlation of human leg muscle fiber orientation by Diffusion Tensor Imaging with creatine s Robert Thomas Engelhardt1, Masahiro Umeda2, Noriaki Hattori3, Chuzo Tanaka2, Tohru Sawada3 1GE Yokogawa Medical Systems, 2Meiji University of Oriental Medicine, 3BF Research Institute 12:51 PM Quantitative Structural Analysis of Rat Lateral Gastrocnemius Fibers Using Diffusion Tensor M Bruce M. Damon1, 2, Zhaohua Ding1, Adam W. Anderson1, and John C. Gore1 1Department of Diagnostic Radiology, Yale School of Medicine, New Haven, CT, USA - 2College of Medicine, University of Illinois, Urbana, IL, USA 12:54 PM Mapping Fiber Orientation in Human Muscle by Short TE MRSI based on Orientation Dependent Dip Peter Vermathen, Roland Kreis, Chris Boesch University & Inselspital, Dept. Clin. Research, AMSM, Bern, Switzerland 12:57 PM Determinants of Anisotropic Intracellular Diffusion of Phosphorous Metabolites in Striated Mu Timothy S. Moerland Department of Biological Science, Florida State University, Tallahassee, Florida 32306-4370 (USA) 1:00 PM Connective tissue MR imaging of muscle samples at 4.7 T J.-M. BONNY, AND J.-P. RENOU SRV / STIM, INRA-Theix, 63122 Saint-Genès-Champanelle, France EMRS IN THE PRESENCE OF SPIN OSCILLATORY MOVEMENTS AS A METHOD FOR PRECISE MEASUREMENT OF 1:03 PM VISCOELASTIC PROPERTIES C.J. Lewa and T. Klinkosz Institute of Experimental Physics, University of Gdańsk, ul. Wita Stwosza 57, 80-952 Gdańsk,

LUNCH 13:30 to 14:30

Thursday, October 4 (Session 3)

PLENARY SESSION :

1

H MR SPECTROSCOPY

Chairmen : R. Kreis, C. Thompson 2:30 PM Potentials and Complications in 1H-MR Spectroscopy of Skeletal Muscle Roland Kreis Department of Clinical Research, MR Unit, University of Bern, MR-Center 1, Inselspital, CH2:55 PM Intramyocellular lipids and insulin resistance Michael Roden, University of Vienna

3:20 PM 1H-MRS, NIRS and insulin CH Thompson Human Nutrition Unit, University of Sydney, NSW, AUSTRALIA 2006

SHORT ORAL COMMUNICATIONS :

1

H MR SPECTROSCOPY

3:45 to 5 : 00 PM High Resolution MR Imaging of Muscular Lipids: Distribution of Lipids in Calf Musculature 3:45 PM in Insulin Resistant and Insulin Sensitive Volunteers F. Schick1, J. Machann1, K. Brechtel1, A. Strempfer1, O.P. Bachmann2, D.B. Dahl2, S. Jacob2, H.U. Häring2, C.D. Claussen1 1Section on Experimental Radiology, Department of Diagnostic Radiology - 2Department of Endocrinology and Metabolism - Eberhard-Karls-University Tübingen, 72076 Tübingen, Germany 3:48 PM Quantification of muscle/fat ratio on MR images of thighs using a fuzzy clustering algorith BARRA V, BOIRE JY Equipe de Recherche en Imagerie Médicale, Faculté de Médecine, Université d'Auvergne, Clermont-Ferrand, France 3:51 PM Effect of charge and stereospecificity in dipolar coupling interactions of lactate and alan Authors : I. Asllani*, E. Shankland**, M. Kushmerick*, **, ***, Departments of Radiology**, Bioengineering* and Physiology & Biophysics***, University of Washington, Seattle, Washington, 98195 3:54 PM

T2 relaxation times of intramyocellular lipid, creatine skeletal muscle at 3T Martin Krssak, Vladimir Mlynarik and Michael Roden

and

water

proton

signals

in

Dpt. of Internal Medicine 3 and Dpt. of Radiology, University of Vienna, Austria 3:57 PM Regulation of intramyocellular lipids (IMCL) under different exogene influences as determin J. Machann1, D.B. Dahl2, K. Brechtel1, O.P. Bachmann2, G. Steidle1, C. Thamer2, S. Jacob2, 1Section on Experimental Radiology, Department of Diagnostic Radiology - 2Department of Endocrinology and Metabolism - Eberhard-Karls-University Tübingen, 72076 Tübingen, 4:00 PM Depletion of intramyocellular lipids during exercise in the m. vastus lateralis, measured b V.B. Hinderling1), L.J.C. van Loon2), W.H.M. Saris2), J.M.A. van Engelshoven1), and M.E. Ko Dept. of Diagnostic Radiology, Academic Hospital of Maastricht, P.O. Box 5800, Maastricht. 4:03 PM In vivo assessment of intramyocellular lipid stores in the flexor digitorum superficialis JA Ward, A Enright, CH Thompson Human Nutrition Unit, Dept of Biochemistry, University of Sydney and IMRR, Sydney, Australi 4:06 PM Skeletal Muscle IMCL and Regional Adiposity: a MRI and MRS Study EL Thomas, TAM Harrington, JD Bell. MRI Unit, Hammersmith Hospital, Du Cane Rd London W12 OHS 4:09 PM Proton MRS Studies of IMCL and EMCL Composition in Healthy Subjects EL Thomas, JD Bell. MRI Unit, Hammersmith Hospital, Du Cane Rd London W12 OHS

COFFEA BREAK AND POSTER VIEWING 5:00 to 5 : 30 PM

Thursday, October 4 (Session 4)

PLENARY SESSION : ANIMAL MODELS Chairmen : A. Heershaap, A. Leroy-Willig 5:30 PM Genetic modification of energy metabolic pathways in mice assessed by MRS A. Heerschap1, K. Renema1, R. in t Zandt1, A. de Groof2, E. Janssen2, F. Oerlemans2, A. Schmidt3, D. Isbrandt3 , B. Wieringa2 1Dept. of Radiology and 2Cell Biology, University Medical Center Nijmegen, the Netherlands. 3Centre for Molecular Neurobiology, University of Hamburg, Germany 5:53 PM Role of NMR for evaluation of gene therapies Anne Leroy-Willig, Institut de Myologie

SHORT ORAL COMMUNICATIONS : ANIMAL MODELS 6:15 to 7:30 PM 6:16 PM Dynamic 31P-NMR measurements of skeletal muscle energetics in wild-type and creatine kinase M.J. Kruiskamp, F. ter Veld, L. Kay, G. van Vliet, and K. Nicolay Department of Experimental in vivo NMR, University Medical Center Utrecht, , The Netherland 31P and 1H MRS reveal differences between brain and muscle metabolism in guanidino acetate 6:19 PM methyltransferase deficient mice W.K.J. Renema1, A. Schmidt2, J.J.A vanAsten1, D. Isbrandt2, K. Ulrich3, B. Wieringa4 and 1Dept. of Radiology and 4Cell Biology, University Medical Center Nijmegen, Nijmegen, the 6:22 PM MR: a useful tool for evaluation of the NO way treatment on mdx mice. B. Gilleta, S. De La Porteb, V. Voisinb, C. Sebriea , J-C. Beloeila a Institut de Chimie des Substances Naturelles, CNRS, Gif sur Yvette, France. b Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, CNRS, Gif sur Yvette, France A Truly Non-Invasive Set-Up for the Determination of Muscle Force Oxidative 6:25 PM Phosphorylations and Perfusion in the Rat Calf in vivo Giovanni Vidal, Claire Wary, Eric Giacomini, and Pierre Carlier NMR Lab, AFM-CEA, Institute of Myology, Pitié-Salpêtrière University Hospital, 83, Bd de l'Hôpital 75651 Paris Cedex 13. Tél: 01 42 16 58 98, fax: 01 42 16 58 94, email : Adaptation of glutamine metabolism in skeletal muscle from aged rats during fasting : a 6:28 PM dynamic 13C NMR study Mezzarobba V., b Bielicki G., b Donnat J.P., a Mignon M., a Grizard J. a Meynial-Denis D an Unité d’Etude du Métabolisme Azoté Centre de Recherche en Nutrition Humaine de ClermontFerrand, 63122 Ceyrat, France, Structure Tissulaire et Interaction Moléculaire, Institut National de la Recherche Agronomique, 63122 Ceyrat, France

6:31 PM 2D MRS monitoring of muscle regeneration C.Sebrie , B.Gillet and J.C. Beloeil ICSN, CNRS, Gif sur Yvette, France

Friday, October 4 (Session 5)

PLENARY SESSION : METABOLIC CHANGES IN CONTRACTING MUSCLE Chairmen : GJ Kemp, K. Conley

3 10

8:15 AM

How theories of metabolic control influence the interpretation of data GJ Kemp Department of Musculoskeletal Science, University of Liverpool L69 3GA, UK.

8:38 AM

Energetic control in muscle: an engineer's perspective. 0 Peter Styles MRC Biochemical and Clinical Magnetic Resonance Unit. Dept. of Biochemistry, University of Oxford, Oxfor

9:01 AM

Role of PCr in aerobic and anaerobic metabolism. Kent Sahlin Adresses : Dept of Physiology and Pharmacology, Karolinska Institutet and Stockholm university College of P.E. and Sports, Sweden.

0

0

SHORT ORAL COMMUNICATIONS : METABOLIC CHANGES IN CONTRACTING MUSCLE 9:15 to 10:30 AM 9:24 AM

Muscle fibre type composition affects muscle ATP turnover at rest in humans Isao Kambayashi1, Aivaras Ratkevicius2, Masao Mizuno3 and Bjørn Quistorff2 1Hokkaido University of Education Sapporo, 5-3-1 Ainosato, Kita-ku, Sapporo, Japan - 2NMR centre, Department of Medical Biochemistry and Genetics A, Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark - 3Department of Anesthesiology, Esbjerg Central

1

9:27 AM

ATP economy of force maintenance in the human tibialis anterior muscle during submaximal voluntary Aivaras Ratkevicius1, Yoshinao Nakagawa1,2, Masao Mizuno1,3 and Bjørn Quistorff1 1NMR centre, Department of Medical Biochemistry and Genetics A, Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark 2Department of Sports and Health Sciences, Otaru University, Otaru, Hokaido 047-0034, Japan 3Department of Anesthesiology, Esbjerg Central Hospital, Østergade 80, 6700 Esbjerg, Denmark

1

9:30 AM

ATP costs of force generation are similar for constant frequency and variable frequency electrical stimulation in human tibialis anterior muscle Aivaras Ratkevicius and Bjørn Quistorff NMR centre, Department of Medical Biochemistry and Genetics A, Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark

1

9:33 AM

Dichloroacetate accelerates the fall in intracellular PO2 at the onset of contractions in Xenopus single1 Richard A. Howlett, Ph.D. and Michael C. Hogan, Ph.D. Dept. of Medicine 0623A, University of California – San Diego, 9500 Gilman Dr., La Jolla, CA 92093-

9:36 AM

Intracellular lactate concentration during intermittend exercise of high intensity 1 ˝N. Maassen+, O. Krückeberg+, G.Schneider++, M. Breyer+, H. Koch*, D. Leibfritz* + Sportsphysiology, Medical School Hannover, FRG ++ MSG-Hannover, FRG * FB II University Breme

9:39 AM

Metabolic determinants of the onset of acidosis in exercising human muscle : a 31P MRS study M. ROUSSEL,1 D. BENDAHAN,1 J.P. MATTEI,1, 2 Y. LE FUR1 AND P.J. COZZONE1 Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine de Marseille, 27, Bd J. Moulin 13005 Marseille -France

1

9:42 AM

Analysis of PCr recovery kinetics after mixed aerobic/glycolytic exercise using 31P MRS GJ Kemp*, M Roussel, D Bendahan, Y Le Fur, PJ Cozzone Centre de Resonance Magnetique Biologique et Medicale, UMR No 6612, Faculté de Médecine, 27 Bd. J. Moulin, 13005 Marseille-France and *Department of Musculoskeletal Science, University of Liverpool

1

9:45 AM

9:48 AM

9:51 AM

Effects of short-duration skeletal muscle exercise on glycogen synthesis during hyperinsulinemia. A 1 study using 13C-Magnetic Resonance Spectroscopy J. H. de Haan*, A.J. van den Bergh*, P. Smits#, C.J.J. Tack+, A. Heerschap* *Dept. of Radiology, +General Internal Medicine, #Clinical Pharmacology University Medical Center Nijmeg Phosphorous-31 relaxation times in human calf muscle: influence of field strength ˝M. Meyerspeer1, M. Krssak2, M. Roden2, E. Moser1,3 1Institut für Medizinische Physik, Universität Wien; 2AKH/Universitätskliniken Institut für Endokrinologie und Stoffwechsel; 3Universitätsklinik für Radiodiagnostik; A-1090 Wien, Austria Identification of Different Metabolic Patterns in Muscles of the Human Calf in 3D-Localized 1HDecoupled 31P MR Spectra 1Truman R. Brown, 1Fernando Arias-Mendoza, and 2Radka Stoyanova 1Columbia University, New York, NY, and 2Fox Chase Cancer Center, Philadelphia, PA

COFFEA BREAK 10:30 to 11:00 AM

1

1

Friday, October 4 (Session 6) PLENARY SESSION AND SHORT ORAL COMMUNICATIONS

MODELISATION Chairmen : M. Kuschmerick, S. Iotti Influence of metal ions concentration on the apparent equilibrium constants of the reactions 11:00 AM involved in the cellular bioenergetics Stefano Iotti Dipartimento di Medicina Clinica e Biotecnologia Applicata -Università di Bologna - ITALY Cytosolic free [Mg2+] in the human calf muscle in different metabolic conditions: in vivo 11:15 AM 31P MRS and computer simulation. S. Iotti , G. Gottardi , B. Barbiroli Dipartimento di Medicina Clinica e Biotecnologia Applicata - Università di Bologna - ITALY Reassessment of the apparent equilibrium constant of Creatine Kinase Reaction for accurate 11:18 AM in vivo assessment of [ADP] by 31P MRS in the human brain and skeletal muscle. Iotti S., Sabatini A*., Vacca A*., Frassineti C§., Barbiroli B. Dipartimento di Medicina Clinica e Biotecnologia Applicata -Università di Bologna - ITALY * Dipartimento di Chimica - Università di Firenze - ITALY - § Dipartimento di Scienze Biomediche - Università di Modena e Reggio Emilia - ITALY

11:31 AM Shaking-up glycolysis: New views on metabolic flux in vivo. Kevin E. Conley, David Marcinek, Will Kemper and Greg Crowther. Departments of Radiology and Physiology & Biophysics. University of Washington Medical Center

Subtrate effects on basal oxygen consumption and the phosphate chemical potential in fast11:46 AM and slow-twitch mouse skeletal muscles at rest Wiseman, Robert W1, JAL Jeneson2 and MJ Kushmerick3 1Molecular Imaging Research Center, Depts Physiology and Radiology, Michigan State University, East Lansing, MI., 2Dept of Pediatrics, University of Utrecht, Utrecht, The Netherlands., 3Depts of Radiology, Physiology and Biophysics, and Bioengineering, 11:49 AM In Vivo Measurements of Mitochondrial Coupling David J. Marcinek, Wayne A. Ciesielski, Kenneth A. Schenkman, Kevin E. Conley Department of Radiology and Department of Pediatrics, University of Washington Medical Center Metabolic Control Analysis of the ATPase network in contracting muscle: regulation of 12:02 PM contractile function and ATP free energy potential J.A.L. Jeneson1,3, H.V. Westerhoff2 and M.J. Kushmerick3,4,5 1Department of Pediatrics, University Medical Center Utrecht, NL, 2Department of Molecular Ce 12:17 PM Integrated kinetic model to quantify energetics in exercising human muscle Martin Kushmerick, Kevin Conley, Gregory Crowther, Sharon Jubrias, Eric Shankland and Paolo V University of Washington, Seattle, WA 98195-7115 12:32 PM Kinetic model for glycogenolysis and glycolysis in skeletal muscle Martin Kushmerick and Melissa Lambeth University of Washington, Seattle, WA 98195-7115

LUNCH 12:45 to 13:45 PM

Friday, October 5 (Session 7)

PLENARY SESSION AND SHORT ORAL COMMUNICATIONS

SKELETAL MUSCLE FUNCTION IN INTEGRATIVE PHYSIOLOGY Chairmen : J. Zange, K. Baldwin 1:45 PM

2 2 Factors Regulating Muscle Mass and Contractile Phenotype in Response to Chronic Alterations in Loadin K.M. Baldwin1, F. Haddad1, G. R. Adams1, V. J. Caiozzo1, R. R. Roy2 and V. R. Edgerton2 Departments of 1Physiology and Biophysics, UC Irvine and 2Physiological Sciences, UC Los Angeles, USA

2:08 PM

Effects of simulated orthostasis on force development, excitability, and energy metabolism in human m Jochen Zange, Sven Baerwalde, Klaus Müller, Norbert Maassen* Institute of Aerospace Medicine, German Aerospace Center, DLR e.V., D-51170 Köln (Cologne), Germany, * Department of Sports Physiology, Medical School Hannover, Germany

2:31 PM

The effect of dietary intake on resting muscle metabolism as measured by NIR spectroscopy C. Ueda, T. Hamaoka, N. Murase, T. Sako, M. Murakami, R. Kime, T. Homma, T. Nagasawa, M. Samejima, S. Ichimura and T. Katsumura Department of Preventive Medicine and Public Health, Tokyo Medical University 6-1-1 Shinjuku-ku, Shinjuku, Tokyo 160-8402, Japan

2:34 PM

Effects of 6 days –6° head down bedrest on the energy metabolism of human calf muscle Jochen Zange, Klaus Müller Institute of Aerospace Medicine, German Aerospace Center, DLR e.V., D-51170 Köln (Cologne), Germany

3:30 to 8:00 PM

FREE AFTERNOON WITH OPTIONAL VISIT OF MARSEILLE 8:00 PM

Conference dinner (optional)

Saturday, October 6 (Session 8)

PLENARY SESSION : COMBINED APPROACHES OF MUSCLE PHYSIOLOGY Chairmen : B. Whipp, K. McCully 8:15 AM

Assigning physiological equivalents to the parameters of VO2 and [PCr] kinetics during high-intensity exercise in humans Brian J. Whipp Department of Physiology, St George's Hospital Medical School, London, SW17 0RE, UK

8:38 AM Multiparametric functional NMR of skeletal muscle : application to in vivo investigations of Pierre Carlier, Institut de Myologie

9:01 AM Evaluating skeletal muscle with light, sound, and radio frequencies. Kevin McCully Address: Department of Exercise Science, University of Georgia, Athens GA USA 9:24 AM 13C NMR spectroscopy of muscle physiology T. Price

9:47 AM Role of muscle produced lactate as substrate for brain and other organs during and after exer Bjørn Quistorff, Mads Dalsgaard, Niels Secher, Aivaras Ratkevicius and Else Danielsen.

SHORT ORAL COMMUNICATIONS : COMBINED APPROACHES OF MUSCLE PHYSIOLOGY 9:45 to 11 : 00 AM 10:10 AM Muscle oxygenation and ATP turnover studied by 31P MRS and NIRS: implications and limitations GJ Kemp1, N Roberts2, WE Bimson2, A Bakran3, SP Frostick1 1Department of Musculoskeletal Science and 2Magnetic Resonance & Image Analysis Research Centre, University of Liverpool, and 3Vascular Unit, Royal Liverpool University Hospital, 10:13 AM

Median frequency, RMS-EMG and contraction speed during dynamic interval handgrip exercise of high intensity V. Shoushacov, N. Maassen Department of the Sports Physiology, Medical School, Hannover, Germany

10:16 AM

Correlation between the spectral analysis of the surface EMG and the mechanical events recorded after chronic electromyostimulation training at the healthy man. MARQUESTE T, DECHERCHI P and JAMMES Y EA 2201 Physiopathologie Respiratoire, Faculté de Médecine Nord, 13916 Marseille cedex 20 EA 3280 Déterminant Physiologique de l’Activité Physique, Faculté des Sciences du Sport,

10:19 AM Surface diaphragmatic electromyogram changes after laparotomy Stéphane V. Berdah1,2 , Robert Picaud 2, and Yves Jammes1 1Laboratoire de Physiopathologie Respiratoire (EA2201), Institut Jean Roche, Faculté de Médecine, Bd Dramard, 13916 Marseille Cedex 20, France 1Service de Chirurgie Digestive, Hôpital Nord, Chemin des Bourrely, 13915 Marseille Cedex 20, France 10:22 AM Biceps Brachii O2Hb De-Saturation and Surface EMG Modifications During Sustained Constant F. Felici1, P. Sbriccoli, I. Bazzucchi1, F. Figura1 M. Ferrari and V. Quaresima University Institute of Motor Sciences, Piazza L. De Bosis 15, 00194, Roma, Italy; Department of Biomedical Sciences and Technologies University of L’Aquila, 67100 L’Aquila, 10:25 AM Correlation between EMG and metabolic data during cycling exercises. HUG F, DECHERCHI P, GRELOT L, JAMMES Y EA 2201 Physiopathologie Respiratoire, Faculté de Médecine Nord, 13916 Marseille cedex 20 EA 3280 Déterminant Physiologique de l’Activité Physique, Faculté des Sciences du Sport, 10:28 AM

10:31 AM

10:34 AM

Dynamic inter-relationships between intramuscular PCr metabolism and pulmonary VO2 during quadriceps exercise in man: Testing superposition using impulse and square-wave inputs H. B. Rossiter1, S. A. Ward3, F. A. Howe2, J. M. Kowalchuk4, J. R. Griffiths2, & B. J. Whipp St. George’s Hospital Medical School, Departments of 1Physiology and 2Biochemistry, University of London, UK; 3Centre for Exercise Science and Medicine, University of Glasgow, UK; 4School of Kinesiology, The University of Western Ontario, London, Canada. Muscle High-Energy Phoshates and Oxygen Metabolism during Electrical Stimulation of Human Quadriceps: a Dynamic Study using Interleaved 1H/31P-NMRS. M. Vanderthommen1, S. Duteil_, J.S. Raynaud_, C. Wary_, A. Leroy-Willig_, J.M. Crielaard1 and 1 Physical Medicine Department, University Hospital, Liège, Belgium; _ NMR laboratory, AFM and CEA, Institute of Myology, Pitié-Salpêtrière Hospital, Paris, France. Noninvasive measurement of muscle oxidative capacity determined by muscle oxygen consumption recovery using NIR spectroscopy T. Nagasawa, T. Hamaoka, N. Murase, T. Sako, M. Murakami, R. Kime, T. Homma, C. Ueda, S. Ichi Department of Preventive Medicine and Public Health, Tokyo Medical University

10:37 AM 18O-assisted 31P NMR: A novel approach to monitor the dynamics of energy metabolism in intact Darko Pucar1, Petras P. Dzeja1, Peter Bast1, Nenad Juranic2, Slobodan Macura2 and Andre Terzi 1Departments of Medicine and Pharmacology and 2Biochemistry and Molecular Biology, Mayo Clinic, Mayo Foundation, 200 First Street SW, Rochester, MN 55905, USA 10:40 AM Captured cellular adenylate kinase-catalyzed phosphotransfer using 18O-assisted 31P NMR Petras P. Dzeja1, Darko Pucar1, Peter Bast1, Nenad Juranic2, Slobodan Macura2 and Andre Terzi 1Departments of Medicine and Pharmacology and 2Biochemistry and Molecular Biology, Mayo Clinic, Mayo Foundation, 200 First Street SW, Rochester, MN 55905, USA 10:43 AM

COFFEA BREAK 11:00 to 11:30 AM

Saturday, October 6 (Session 9)

PLENARY SESSION : FATIGUE, TRAINING, CREATINE SUPPLEMENTATION Chairmen : K. Sahlin, B. Quistorff

The reduction in ATP cost of contraction throughout fatiguing protocol is independent of the 11:45 AM fatigue level. An in vivo study in stimulated rat gastrocnemius muscle Benoît Giannesini, Marguerite Izquierdo, Yann Le Fur, Patrick J. Cozzone and David Bendahan Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine de Marseille, 27, Bd J. Moulin 13005 Marseille -France 12:08 PM Fatigue after low-intensity stretch-shortening cycle exercise Guillaume Y. MILLET, Vincent MARTIN, Nicola A. MAFFIULETTI Groupe Analyse du Mouvement, Faculté des Sciences du Sport, BP 27877, 21078 Dijon cedex, France

SHORT ORAL COMMUNICATIONS : FATIGUE, TRAINING, CREATINE SUPPLEMENTATION 12:00 to 1:00 PM Thigh muscle volumes and proton T2 relaxation time after reconstruction of the anterior cruciate 12:31 PM ligament and 12 weeks of rehabilitation Haithem Rebai1, Vincent Barra2, Jean-Marie Bonny3, Georges Poumarat4 and Jean Coudert1 Laboratoire de Physiologie-Biologie du Sport1, Equipe de recherche en Imagerie Médicale2, Laboratoire d'Anatomie et d’Organogenèse4, Faculté de Médecine, Université d'Auvergne, Institut 12:34 PM The effects of soleus electrostimulation training on the plantarflexor and dorsiflexor voluntary Nicola A. MAFFIULETTI, Alain MARTIN, Manuela PENSINI Laboratoire Groupe Analyse du Mouvement, Faculté de Sciences du Sport – UFR STAPS, BP 27877 – 21078 Dijon cedex, France

12:37 PM

Changes of muscle excitibility during dynamic forearm exercise of moderate intensity Mareike Fenner, Vladimir Shushakov, Norbert Maassen Institute of Aerospace Medicine, German Aerospace Center, DLR Department of Sports Physiology, Medical School Hannover, Germany

e.V.,

D-51170

Köln,

Germany,

Indirect effect of inorganic phosphate on force production evidenced by in vivo 31P-MRS in rat 12:40 PM gastrocnemius muscle B. Giannesini, M. Izquierdo, P.J. Cozzone and D. Bendahan. Centre de Résonance Magnétique Biologique et Médicale (CRMBM), UMR CNRS 6612, Faculté de Médecine de Marseille, 27 Boulevard Jean Moulin, 13005 Marseille, France. 12:43 PM Creatine supplementation results in elevated PCr/ATP ratios in the calf muscle of athletes but not J. Zange 1, K. Müller 1, S. Kurtscheid 2, H. Heck 2, C. Kornblum 3, R. Schröder 3, T. Grehl 4 , M 1 German Aerospace Center (DLR), Cologne, Germany; 2 Institute of Sportsmedicine, Ruhr-University Bochum, Germany; 3 Departments for Neurology, University Bonn, Germany; 4 Departments for Neurology, Ruhr-University Bochum; Germany 12:46 PM Creatine supplementation affects ATP production rate during maximalExercise Yuko Kurosawa, Takafumi Hamaoka and Toshihito Katsumura Department of Preventive Medicine and Public Health, Tokyo Medical University6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan

Energy transients in skeletal muscle using 31P Magnetic Resonance Spectroscopy. A comparative 12:49 PM study of sportive and sedentary populations during an exhaustive short lasting forearm exercise O.Brosseau (1), R.Mahdjoub (1), MJ.Seurin (1), D.Graveron-Demilly (1), P.Thiriet (2) D.Gozal (3), (1)Laboratoire de Résonance Magnétique Nucléaire, Instrumentation et Méthodologie en Biophysique. UMR 5012 CNRS. Domaine scientifique de la Doua, CPE, 3 rue Victor Grignard, 69616 Villeurbanne France.(2)Centre de Recherche et d’Innovation sur le Sport, EA 647 UCBL. Domaine scientifique de

LUNCH AND POSTER VIEWING 13:00 - 2:30 PM

Saturday, October 6 (Session 10)

PLENARY SESSION : NON INVASIVE INVESTIGATION OF MUSCLE DISEASES Chairmen : D. Bendahan, R. Lodi 2:30 PM Functional evaluation of metabolic myopathies during exercise: a role for NIRS? B. Grassi1,3, L. Morandi2, S. Pogliaghi1,3, S. Rampichini3, C. Marconi3, and P. Cerre 1) Dip. Scienze e Tecnologie Biomediche, School of Medicine, University of Milano, Milano (Italy) (e-mail [email protected]); 2) Istituto Neurologico “Carlo Besta”, Milano; 3) Sezione di Fisiologia Molecolare e Biotecnologie, CNR, Milano. 2:53 PM Near infrared spectroscopy applied to metabolic myopathies: a combined MRS and NIRS a Willy NJM Colier1,2, Mireille CP van Beekvelt2,3, Ron Wevers3, , Jochen Zange4, Mathias Vorgerd5, Valentina Quaresima6, Marco Ferrari6, Baziel van Engelen3 1Dept. of Geriatric Medicine, 2Physiology and 3Neuromuscular Centre Nijmegen, UMC Nijmegen, The Netherlands; 4DLR, Cologne, Germany; 5Dept. of Neurology, Ruhr University, Bochum, Germany; 6L’Aquila Medical School, Italy. 3:16 PM

Physiological, biochemical and genetic effects of exercise and training in mitochond Tanja Taivassalo*, Eric Shoubridged, Douglas Arnoldd, Ronald Haller* * Institute for Exercise and Environmental Medicine, Dallas, Texas 75231, Montreal Neurological Institute, McGill University, Montreal, Quebec, H3A2B4,

3:39 PM Friedreich's ataxia a "new" mitochondrial disorder: from pathogenesis to therapy pros Raffaele Lodi Dipartimento di Medicina Clinica e Biotecnologia Applicata, Universita’ di Bologna, B

SHORT ORAL COMMUNICATIONS OF MUSCLE DISEASES

: NON INVASIVE INVESTIGATION

3:30 to 4:30 AM A Simple In Vivo 31P MR Spectroscopy Protocol to Study Active Muscle Non-Invasively 4:02 PM in Pediatric Patients with Neuromuscular Disease Fernando Arias-Mendoza1, Mary A. Selak2, Joseph Melvin3, Leon Salganicoff3, Warren D. Grover3, Edward Kaye2, and Truman R. Brown1. Columbia University1, New York City, NY, USA, and Children Hospital of Philadelphia2, and St. Christopher’s Hospital for Children3, Philadelphia, PA, USA. New Parameters Reducing the Interindividual Variability of Metabolic Changes during 4:05 PM Muscle Contraction in Humans. A P-31 MRS Study with Clinical Implications. JP Mattei, D Bendahan, M Roussel, G Kozak-Ribbens, Y Le Fur, PJ Cozzone Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine de Marseille, 27, Bd J. Moulin 13005 Marseille -France Family Creatine Deficiency with a Myophatic Component: Near-Absent Signals in the 1H4:08 PM decoupled 31P Magnetic Resonance Spectra of Calf Muscle. F. Arias-Mendozaa, M. Selakb, L. Konchaninb, W. D. Groverc, & T. R. Browna. Columbia Universitya, New York City, NY, USA, and St. Christopher’s Hospital for Childrenb, and Children Hospital of Philadelphiac, Philadelphia, PA, USA. 31P MRS for Malignant Hyperthermias (from the characterization of metabolic 4:11 PM abnormalities to a tool for diagnosis) D. Bendahan, G. Kozak-Ribbens, B. Ghattas, S. CONFORT-Gouny, P.j. Cozzone Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine de Marseille, 27, Bd J. Moulin 13005 Marseille -France 4:14 PM Is muscle metabolism and blood flow impaired in chronic fatigue syndrome? Sinclair Smith, Kevin McCully, Benjamin Natelson, Addresses: Temple University, University of Georgia, New Jersey School of Medicine and Dentistry, University of Pennsylvania : 31P-MRS demonstrates a tissue specific distribution of the in vivo biochemical 4:17 PM expression of G3460A Leber's Hereditary Optic Neuropathy mtDNA mutation. R. Lodi1, P. Montagna2, S.Iotti1,V. Carelli2, M.L. Valentino2, B. Barbiroli1 1 Dipartimento di Medicina Clinica e Biotecnologia Applicata “D. Campanacci”; 2 Istituto di Clinica Neurologica, Universita’ di Bologna, Italy 4:20 PM QUANTITATIVE MRI AND MUSCLE FUNCTION STUDIES IN INCLUSION BODY MYOSITIS (IBM) FL Mastaglia1,2, BA Phillips3, LA Cala4, GW Thickbroom1, PJ Zilko5 Centre for Neuromuscular and Neurological Disorders1 and ARCME4 University of Western Australia1; Departments of Neurology and Clinical Neurophysiology2 and Clinical Immunology5, Queen Elizabeth II Medical Centre, Perth; School of 4:23 PM Heparin Dose and Lower Leg Muscle Cross Sectional Area in Dialysis Patients Giorgos K Sakkas 1*, Tiffany Shubert 2, Julie Doyle 2, Jane A. Kent-Braun 3 and 1Department of Medicine, University of California San Francisco, San Francisco, CA; 2Nephrology Section, San Francisco VA Medical Center, San Francisco, CA; and 3University of Massachusetts, Amherst, MA. 4:26 PM Evaluation of muscular glycogen content by 13C NMR spectroscopy in adult-onset GSD C. Wary, P. Laforêt1, B. Eymard1, M. Fardeau1, A. Leroy-Willig, P.G. Carlier NMR Laboratory- AFM- CEA- Institute of Myology, Pitie-Salpetriere Hospital, Paris, France, 1Institute of Myology, AFM, Pitie-Salpetriere Hospital, Paris, France

COFFEA BREAK AND POSTER VIEWING 05:00 - 6:0 PM

CONCLUDING REMARKS 6:30 PM

FAREWELL GASTRONOMIC PARTY (OPTIONAL) 8:00 PM