Peroxidase immobilized on Amberlite IRA-743 resin for on-line
Feb 3, 2006 - spectrophotometric detection of hydrogen peroxide in rainwater ... peroxide (H2O2) in the oxidation of SO2 and other compounds has been well established. ... The availability of H2O2 in both the gas phase and the aque-.
Peroxidase immobilized on Amberlite IRA-743 resin for on-line spectrophotometric detection of hydrogen peroxide in rainwater Renato Camargo Matos ∗ , Eunice Oliveira Coelho, Cabrini Ferraz de Souza, Felipo Amaral Guedes, Maria Auxiliadora Costa Matos N´ucleo de Pesquisa em Instrumenta¸ca˜ o e Separa¸co˜ es Anal´ıticas (NUPIS), Departamento de Qu´ımica, Instituto de Ciˆencias Exatas, Universidade Federal de Juiz de Fora, 36038-300 Juiz de Fora, MG, Brazil Received 8 November 2005; received in revised form 15 December 2005; accepted 16 December 2005 Available online 3 February 2006
Abstract The importance of atmospheric hydrogen peroxide (H2 O2 ) in the oxidation of SO2 and other compounds has been well established. A spectrophotometric method for the determination of hydrogen peroxide in rainwater is proposed. This method is based on selective oxidation of hydrogen peroxide using an on-line tubular reactor containing peroxidase immobilized on Amberlite IRA-743 resin. The hydrogen peroxide in the presence of phenol, 4-aminoantipyrine and peroxidase, produces a red compound (λ = 505 nm). Beer’s law is obeyed in a concentration range of 1–100 mol l−1 hydrogen peroxide with an excellent correlation coefficient (r = 0.9991), at pH 7.0, with a relative standard deviation (R.S.D.)
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