Morphology • Gram-positive cocci, 0.5-0.75 µm • Arranged in chains, usually 10 or more, but for S. pneumoniae usually as diplococci • Catalase negative • Non-motile, except 2 Enterococcal species • Facultative anaerobic • Enriched media • Colonies on blood agar are either – -haemolytic, – - haemolytic, or – non-haemolytic
Gram-positive cocci in chains
Classification • • • •
Lancefield 1930 (A.B.C…….) ß- Hemolysis (A.B.C & some D strains) α-hemolysis( St.pneumonia & Viridans St.) Nonhemolytic( nonpathogen)
Beta hemolysis
Alpha hemolysis
St.pyogenes (ß-hemolytic streptococci) • Group A • Throat ,Nasopharynx and skin
Post streptococcal infections group A • Rheumatic fever After tonsillitis or pharyngitis ( carditis,abnormal ECG, painful arthritis, chorea and fever) • Acute glumerulonephritis
Group D streptococci and Enterococci • • • • •
E.faecium E.faecalis(UTI,endocarditis,speticemia) E.durans St.equinus Bacteremia and endocarditis St.bovis
Streptococcus agalactia(group B) • Puerperal, neonatal, ulceral and skin infections. susceptible: Diabetes and vascular disesases
Laboratory diagnosis • Rapid test ( monoclonal antibodies Kits) • Cultivation on sheep blood agar or enrichment media • Beta hemolysis group A(C,G,D) • ASLO
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