DEVELOPMENT OF A DETECTION AND ... - Laboratoire BioSol
1 Laboratoire BioSol- 13 rue du Nord 76000 Rouen-France. 2 A.BIO.C- Route ... relevance of DNA extraction and molecular detection and quantification methods. The ... euteiches in alfalfa with a fluorescent polymerase chain reaction assay.
DEVELOPMENT OF A DETECTION AND QUANTIFICATION METHOD OF APHANOMYCES EUTEICHES FRENCH ISOLATES IN SOIL. H.Sauvage(1,2) K.Laval (1) F.Bois(2) S.Barray(3) 1
Laboratoire BioSol- 13 rue du Nord 76000 Rouen-France A.BIO.C- Route de Samadet 64410 Arzacq- France 3 Laboratoire de Microbiologie du Froid- UFR Sciences, Université de Rouen 76130 Mont Saint Aignan - France 2
Aphanomyces euteiches, the cause of root rot of peas (Pisum sativum) is the most destructive soilborne disease of peas. It has started to cause serious troubles in France because of intensification of the pea production. An effective method of fighting and removal of the disease is not yet applicable in the case of this major plant pathogen. The only effective way consists of appreciating inoculum level in the soil in order to avoid strongly infested grounds. A quantitative real-time assay using TaqMan chemistry has been developed to quantify the level of Aphanomyces euteiches contamination in soil. The results presented are under framework whose objective aims at proposing a tool for diagnosis (I) The specificity of primer/probe set proposed by Vandemark[1, 2] has been validated on French isolates. (II) In order to be able to compare samples between them, we determinated the number of copies of target (76pb) on Aphanomyces euteiches genome and checked that this number was constant whatever isolates present. (III) Correlation has been established between the number of artificially inoculated oospores and root necrosis quantity after 14 days incubation. The molecular tool has been then transfered to naturally infested ground in order to appreciate the relevance of DNA extraction and molecular detection and quantification methods. The molecular tool could thus be an advantage in terms of decision-making for growers and the method could be then applied to many plant pathogens in order to prevent productions from significant damages.
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Vandemark G.J., et al., A PCR-based assay by sequence-characterized DNA markers for the identification and detection of aphanomyces euteiches. Phytopathology, 2000. 90(10): p. 1137-1144. Vandemark G.J., Barker B.M., and Gritsenko M.A., Quantifying aphanomyces euteiches in alfalfa with a fluorescent polymerase chain reaction assay. The American phytopathological society, 2002. 92(3): p. 265-272.
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microbial carbon analysis, total quantification of extracted DNA and enumeration of cultivable ... Genetic fingerprints are in progress.Since impacts of sewage ...
and total DNA assay. The bacteria content has been approached ... Moreover, genetic fingerprints complexity is related to the age of the meadow suggesting an ...
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Element content was determined by GF-AAS. All these measurements enable to understand the transfers of TEs from soil to flax. Keywords: soil/plant transfer, ...
TE concentrations of the different parts of plants show their uptake and their distribution from roots to seeds. Elemental contents were determined by GF-AAS.
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