454 pyrosequencing of foliar fungal communities ... - tristan cordier
data : ideas and limits. Altitude or latitude. Species richness. A, B, C. A, C, D ... Index Fungorum (synonyms). â Fungal database USDA. â GenBank. 3. 0. 7. 0.
Main objective: Effect of climate change on functional diversity
Main method: New samplings along altitudinal transects
454 pyrosequencing of foliar fungal communities associated with beech along an altitudinal gradient Cordier T., Capdevielle X., Robin C., Desprez-Loustau ML., & Vacher C.
What is the effect of climate on foliar fungal communities richness and composition? Species richness.
C, D, E A, C, D A, B, C
Altitude or latitude
How to estimate functional diversity from these data : ideas and limits symbiont C A B
parasite
saprobe
1
18/05/2011
Sampling
Abundance 6°C gradient
OTU
25 meters minimum between sampling unit 14
Temperature (°C)
13 12
Distribution pattern
11
MEGAN
10 9 8 Annual mean temperature 7 1600
1200
800
400
Altitude (meters)
100
June
July
September
Preliminary Results
71175 high quality reads 800 Operational Taxonomic Units (OTU) supported by at least 2 reads and 865 singletons with plutoF (Tedersoo, Nilsson et al., 2010) Within these 1695 OTU, 215 are identified at the species level (> 97% similarity on a reference sequence, GenBank, UNITE) Few dominant OTU and many rare OTU
2
18/05/2011
Richness pattern Adjusted OTU richness
GLM : Richness = Sampling Date + Temperature + Precipitation No sampling date effect and no temperature effect (p>0.5) but a precipitation effect (p
The 454 sff files are available from the European Nucleotide Archive (http:// ...... document] URL http://drive5.com/usearch [accessed 20 May 2012]. Fauth JE .... software package for automated extraction of ITS1 and ITS2 from fungal ITS.
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Springer Science+Business Media New York 2016. Keywords Microbial communities ... suring tens of micrometers across) [9], we hypothesized that (i) like fungi, ...
The distance-decay pattern of similarity in ecological communities has been recognized by ecologists for de- cades [1]. Such a pattern has been shown for ...
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How to share the specific expertise of each particular grass root community groups. How to create with it a bank of knowledge (on legal issues, planning issues ...
the purposes of taxonomic identification. We applied a threshold of at least 97 % similarity over at least 90 % of the query length of a fully annotated accession ...
